Science and Education

NET-Seq

LAM-HTGTS

LAM-HTGTS is a method for the genome-wide detection of “prey” DSBs via their translocation to a fixed “bait” DSB in cultured mammalian cells. Bait-prey junctions are cloned directly from isolated gDNA using LAM-PCR and ligated unidirectionally to bridge adapters. Subsequent PCR steps amplify the single-stranded DNA junction library in preparation for sequencing..

Pros:
  • Sensitive detection of DSBs within chromosomes
Cons:
  • Not applicable to limited amounts of material
  1. Hu J., Meyers R. M., Dong J., Panchakshari R. A., Alt F. W., et al. Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing. Nat Protoc. 2016;11:853-871
For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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