TruSeq Rapid Exome Library Prep Kit

This exome sequencing library prep kit uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome. Read More...
Select Product(s)

TruSeq Rapid Exome (8 rxn x 1 plex)



TruSeq® Rapid Exome Kit (8 rxn × 3 plex)



TruSeq® Rapid Exome Kit (8 rxn × 6 plex)



TruSeq® Rapid Exome Kit (8 rxn × 9 plex)



TruSeq® Rapid Exome Kit (8 rxn × 12 plex)



Product Highlights

The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

  • Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
  • Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
  • High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality

The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit


Method-Specific Workflow Example


Supporting Data and Figures

Consistent Tagmentation for Varying Input Amounts
TruSeq Rapid Exome Library Prep Kit graph

Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results.

TruSeq Rapid Exome Library Prep Kit Configurations
Catalog Number TruSeq Rapid Exome Library Prep Kit Total Samples Number of Enrichment Reactions Number of Samples per Enrichment Reaction Unique index adapter combinations
FC-144-1000 8 rxn × 1 plex 8 8 1 1
FC-144-1001 8 rxn × 3 plex 24 8 3 3
FC-144-1002 8 rxn × 6 plex 48 8 6 6
FC-144-1003 8 rxn × 9 plex 72 8 9 9
FC-144-1004 8 rxn × 12 plex 96 8 12 24

Product Literature

Illumina Exomes Comparative Table

Product Information Sheet | PDF(< 1 MB)

TruSeq Rapid Exome Library Prep Kit

Data Sheet | PDF(< 1 MB)

Manuals and Support Information

TruSeq Rapid Exome Library Prep Kit Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Rapid Exome Library Prep Kit Support 

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Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.

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TruSight One Sequencing Panels

Comprehensive panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.