Nextera DNA Exome offers two configuration options. The product previously known as the TruSeq Rapid Exome Library Prep Kit (Cat. No. FC-144-1000, FC-144-1001, FC-144-1002, FC-144-1003, and FC-144-1004) is now called the Nextera DNA Exome Kit and is available as a pre-assembled kit. Kits include Illumina library preparation reagents, index adapters, enrichment reagents, and an exome panel. Alternatively, to combine Illumina Nextera DNA Exome library preparation with IDT xGen exome enrichment, major components (such as library preparation and index adapters) can be purchased separately. A full list of components available for individual purchase is shown on the “Choose Your Components” tab below. IDT xGen exome enrichment reagents may be purchased from IDT.

Nextera DNA Exome

This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.Read More...
Select Product(s)
What products do I need?

Nextera™ Exome Kit (24 Samples)



Nextera™ Exome Kit (96 Samples)


Library Prep

Nextera™ DNA Library Prep for Enrichment (24 Samples)



Nextera™ DNA Library Prep for Enrichment (96 Samples)



Index Adapters

Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)



Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)


Accessory Products
What accessories do I need?

Illumina Free Adapter Blocking Reagent (12 reactions)



Illumina Free Adapter Blocking Reagent (48 reactions)



Product Highlights

The Nextera Exome Kit, previously known as the TruSeq Rapid Exome Library Prep Kit, delivers a simple, efficient method for high-confidence calling of exonic variants. This fast library preparation and exome enrichment workflow delivers libraries in less than 2 days with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. Benefits include:

  • Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment
  • Rapid, automation-friendly workflow completes in less than 2 days with only 3 hours of hands-on time
Proven Nextera Data Quality

The Nextera Exome Kit delivers an average of >75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Illumina now offers modular product ordering to enable flexibility in your workflows.

Find a list of automation vendors with robotic systems compatible with this solution

Illumina–IDT Exome Enrichment Solution

Illumina has partnered with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome, and covers 51 Mb of end-to-end tiled space.
xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries; contact IDT for Nextera libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.

Learn more about the IDT xGen Exome Research Panel
Illumina and IDT Partner on NGS Multiplexing and Exome Enrichment


Method-Specific Workflow Example


Supporting Data and Figures

Consistent Tagmentation for Varying Input Amounts
TruSeq Rapid Exome Library Prep Kit graph

Y-axis: Fluorescence Units (FU), X-axis: Base Pairs (bp)


Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results.

Nextera Exome Kit (Pre-Assembled Kits)
Catalog Number Total Samples Number of Enrichment Reactions Index Adapters
20020616 24 8 24 combinatorial dual indexes
20020617 96 8 96 combinatorial dual indexes

Nextera DNA Exome (formerly known as TruSeq Rapid Exome) is the recommended replacement product for the Nextera Rapid Capture Exome and Expanded Exome Kits, which have been discontinued. Illumina remains committed to providing you with high-quality support and service.

Related Products