|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data)||2 × 75 bp (max recommended)|
|HiSeq 2500 System||Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data)||2 × 75 bp (max recommended)|
|NovaSeq 6000 System||Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (based on 50 million reads, 50× coverage, 4 Gb of data)||≤ 2 × 100 bp|
|Nextera DNA Exome||TruSeq DNA Exome||AmpliSeq for Illumina Exome Panel||Nextera Flex for Enrichment|
|Assay Time||< 2.5 days||2.5 days||< 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)||~6.5 hours|
|Content Specifications||Captures coding exons. Spans a 39 Mb target region and covers 51 Mb of end-to-end tiled space.||Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).||> 97% of CCDS||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Description||A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.||A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.||Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.|
|Hands-On Time||3 hours||6 hours||< 1.5 hours||~2 hours|
|Input Quantity||50 ng genomic DNA||100 ng genomic DNA||50 ng minimum||10-1000 ng high-quality genomic DNA or ≥ 50 ng FFPE DNA. (For blood and saliva, see the reference guide).|
|Mechanism of Action||Transposase-based fragmentation and exome enrichment with biotinylated capture probes||Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes||Multiplex PCR||Enrichment-compatible, bead-linked transposome|
|Method||Exome Sequencing||Exome Sequencing||Amplicon Sequencing , Exome Sequencing||Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 12-plex enrichment||Up to 12-plex enrichment||96 dual index combinations||Up to 96 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.|
|On-Target Reads||>75%||>80%||>80%||≥85% (for exome panel)|
|Specialized Sample Types||Low-Input Samples, Not FFPE-Compatible||FFPE Tissue||Not FFPE-Compatible||Blood, FFPE Tissue, Saliva|
|Species Category||Human||Human||Human||Human, Other|
|Uniformity||>75% (% coverage at 20x for 4 Gb)||>85% (% coverage at 20x for 4 Gb)||>90% (% coverage at 20x for 4 Gb)||≥90% (% coverage at 20x for 4 Gb; for exome panel)|
Y-axis: Fluorescence Units (FU), X-axis: Base Pairs (bp)
Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results.
|Catalog Number||Total Samples||Number of Enrichment Reactions||Index Adapters|
|20020616||24||8||24 combinatorial dual indexes|
|20020617||96||8||96 combinatorial dual indexes|