Nextera DNA Exome offers two configuration options. The product previously known as the TruSeq Rapid Exome Library Prep Kit (Cat. No. FC-144-1000, FC-144-1001, FC-144-1002, FC-144-1003, and FC-144-1004) is now called the Nextera DNA Exome Kit and is available as a pre-assembled kit. Kits include Illumina library preparation reagents, index adapters, enrichment reagents, and an exome panel. Alternatively, to combine Illumina Nextera DNA Exome library preparation with IDT xGen exome enrichment, major components (such as library preparation and index adapters) can be purchased separately. A full list of components available for individual purchase is shown on the “Choose Your Components” tab below. IDT xGen exome enrichment reagents may be purchased from IDT.

Nextera DNA Exome

This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.Read More...
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What products do I need?

Nextera™ Exome Kit (24 Samples)

20020616

Price
 
 

Nextera™ Exome Kit (96 Samples)

20020617

Price
 
 
Library Prep

Nextera™ DNA Library Prep for Enrichment (24 Samples)

20020187

Price
 
 

Nextera™ DNA Library Prep for Enrichment (96 Samples)

20020188

Price
 
 

Index Adapters

Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)

20018707

Price
 
 

Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)

20018708

Price
 
 
Accessory Products
What accessories do I need?

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Price
 
 

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Price
 
 

Product Highlights

The Nextera Exome Kit, previously known as the TruSeq Rapid Exome Library Prep Kit, delivers a simple, efficient method for high-confidence calling of exonic variants. This fast library preparation and exome enrichment workflow delivers libraries in less than 2 days with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. Benefits include:

  • Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment
  • Rapid, automation-friendly workflow completes in less than 2 days with only 3 hours of hands-on time
Proven Nextera Data Quality

The Nextera Exome Kit delivers an average of >75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Illumina now offers modular product ordering to enable flexibility in your workflows.

Find a list of automation vendors with robotic systems compatible with this solution

Illumina–IDT Exome Enrichment Solution

Illumina has partnered with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome, and covers 51 Mb of end-to-end tiled space.
xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries; contact IDT for Nextera libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.

Learn more about the IDT xGen Exome Research Panel
Illumina and IDT Partner on NGS Multiplexing and Exome Enrichment

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data) 2 × 75 bp (max recommended)
HiSeq 2500 System Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data) 2 × 75 bp (max recommended)
NovaSeq 6000 System Samples per run (dual flow cell):   S1: 96, S2: 96, S4: 192  (based on 50 million reads, 50× coverage, 4 Gb of data) ≤ 2 × 100 bp

Product Comparison

Nextera DNA Exome TruSeq DNA Exome AmpliSeq for Illumina Exome Panel Nextera Flex for Enrichment
Assay Time < 2.5 days 2.5 days < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) ~6.5 hours
Content Specifications Captures coding exons. Spans a 39 Mb target region and covers 51 Mb of end-to-end tiled space. Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). > 97% of CCDS Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Description A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.
Hands-On Time 3 hours 6 hours < 1.5 hours ~2 hours
Input Quantity 50 ng genomic DNA 100 ng genomic DNA 50 ng minimum 10-1000 ng high-quality genomic DNA or ≥ 50 ng FFPE DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes Multiplex PCR Enrichment-compatible, bead-linked transposome
Method Exome Sequencing Exome Sequencing Amplicon Sequencing , Exome Sequencing Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing
Multiplexing Up to 12-plex enrichment Up to 12-plex enrichment 96 dual index combinations Up to 96 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.
On-Target Reads >75% >80% >80% ≥85% (for exome panel)
Specialized Sample Types Low-Input Samples, Not FFPE-Compatible FFPE Tissue Not FFPE-Compatible Blood, FFPE Tissue, Saliva
Species Category Human Human Human Human, Other
Uniformity >75% (% coverage at 20x for 4 Gb) >85% (% coverage at 20x for 4 Gb) >90% (% coverage at 20x for 4 Gb) ≥90% (% coverage at 20x for 4 Gb; for exome panel)

Method-Specific Workflow Example

 

Supporting Data and Figures

Consistent Tagmentation for Varying Input Amounts
TruSeq Rapid Exome Library Prep Kit graph

Y-axis: Fluorescence Units (FU), X-axis: Base Pairs (bp)

 

Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results.

 
Nextera Exome Kit (Pre-Assembled Kits)
Catalog Number Total Samples Number of Enrichment Reactions Index Adapters
20020616 24 8 24 combinatorial dual indexes
20020617 96 8 96 combinatorial dual indexes

Nextera DNA Exome (formerly known as TruSeq Rapid Exome) is the recommended replacement product for the Nextera Rapid Capture Exome and Expanded Exome Kits, which have been discontinued. Illumina remains committed to providing you with high-quality support and service.

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