Nextera DNA Exome offers two configuration options. The product previously known as the TruSeq Rapid Exome Library Prep Kit (Cat. No. FC-144-1000, FC-144-1001, FC-144-1002, FC-144-1003, and FC-144-1004) is now called the Nextera Exome Kit and is available as a pre-assembled kit. Kits include all Illumina reagents for library preparation, index adapters, enrichment reagents, and an exome panel. To enable the combination of the Illumina Nextera DNA Exome library preparation with IDT’s xGen exome enrichment, major components (such as library preparation and index adapters) can also be purchased separately. A full list of components available for individual purchase can be found on the “Choose Your Components” tab. Reagents required for IDT’s xGen exome enrichment can be purchased by contacting IDT.

Nextera DNA Exome

This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome. Read More...
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Nextera® Exome Kit (24 Samples)



Nextera® Exome Kit (96 Samples)


Library Prep

Nextera® DNA Library Prep for Enrichment (24 Samples)



Nextera® DNA Library Prep for Enrichment (96 Samples)



Index Adapters

Nextera DNA CD Indexes (24 Indexes, 24 Samples)



Nextera DNA CD Indexes (96 Indexes, 96 Samples)



Product Highlights

The Nextera Exome Kit, previously known as the TruSeq Rapid Exome Library Prep Kit, delivers a simple, efficient method for high-confidence calling of exonic variants. This fast library preparation and exome enrichment workflow delivers libraries in less than 2 days with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. Benefits include:

  • Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment
  • Rapid, automation-friendly workflow completes in less than 2 days with only 3 hours of hands-on time
Proven Nextera Data Quality

The Nextera Exome Kit delivers an average of >75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Illumina now offers modular product ordering to enable flexibility in your workflows.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this workflow

Illumina–IDT Exome Enrichment Solution

Illumina is partnering with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel v1.0, which consists of 429,826 individually synthesized, and quality controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled space. xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries; contact IDT for Nextera libraries), and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.

Learn more about the IDT xGen Exome Research Panel v1.0
Illumina and IDT Partner on NGS Multiplexing and Exome Enrichment


Method-Specific Workflow Example


Supporting Data and Figures

Consistent Tagmentation for Varying Input Amounts
TruSeq Rapid Exome Library Prep Kit graph

Y-axis: Fluorescence Units (FU), X-axis: Base Pairs (bp)


Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results.

Nextera Exome Kit (Pre-Assembled Kits)
Catalog Number Total Samples Number of Enrichment Reactions Index Adapters
20020616 24 8 24 combinatorial dual indexes
20020617 96 8 96 combinatorial dual indexes

Product Literature

Exomes Comparative Table,Illumina Exomes Comparative Table

Product Information Sheet | PDF < 1 MB

Manuals and Support Information

Nextera DNA Exome Support - Documentation & Literature

TruSeq Rapid Exome Library Prep Kit Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All Nextera DNA Exome Support 

All TruSeq Rapid Exome Library Prep Kit Support 

Related Products

TruSeq DNA Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.

Nextera Rapid Capture Custom Enrichment Kit

Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.

TruSight One Sequencing Panels

Comprehensive panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.