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Nextera DNA Exome offers two configuration options. The product previously known as the TruSeq Rapid Exome Library Prep Kit (Cat. No. FC-144-1000, FC-144-1001, FC-144-1002, FC-144-1003, and FC-144-1004) is now called the Nextera DNA Exome Kit and is available as a pre-assembled kit. Kits include Illumina library preparation reagents, index adapters, enrichment reagents, and an exome panel. Alternatively, to combine Illumina Nextera DNA Exome library preparation with IDT xGen exome enrichment, major components (such as library preparation and index adapters) can be purchased separately. A full list of components available for individual purchase is shown on the “Choose Your Components” tab below. IDT xGen exome enrichment reagents may be purchased from IDT.
The Nextera Exome Kit, previously known as the TruSeq Rapid Exome Library Prep Kit, delivers a simple, efficient method for high-confidence calling of exonic variants. This fast library preparation and exome enrichment workflow delivers libraries in less than 2 days with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. Benefits include:
The Nextera Exome Kit delivers an average of >75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels but still achieves uniform coverage for high-confidence results.
To learn more about calculating coverage estimates, see the sequencing coverage calculator.
Illumina now offers modular product ordering to enable flexibility in your workflows.
Find a list of automation vendors with robotic systems compatible with this solution
Illumina has partnered with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome, and covers 51 Mb of end-to-end tiled space.
xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries; contact IDT for Nextera libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.
Y-axis: Fluorescence Units (FU), X-axis: Base Pairs (bp)
Enhanced transposome chemistry is highly tolerant of varying DNA input amounts, reducing bias and providing consistent results.
| Catalog Number | Total Samples | Number of Enrichment Reactions | Index Adapters |
|---|---|---|---|
| 20020616 | 24 | 8 | 24 combinatorial dual indexes |
| 20020617 | 96 | 8 | 96 combinatorial dual indexes |
Product Information Sheet | PDF < 1 MB
Data Sheet | PDF | 5 versions
Nextera DNA Exome Support - Documentation & Literature
Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.
Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.
Comprehensive panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.
Targeted research panel for sequencing the protein-coding regions of the human genome.
