scTrio-Seq
scTrio-Seq
scTrio-Seq can analyze genomic CNVs, the DNA methylome, and the transcriptome of an individual mammalian cell simultaneousl. This approach is an extension of previous methods, such as scMT-seq.
Pros:
- Accurately analyzes the mechanism by which the transcriptome, genome, and DNA methylome regulate each other
- CNVs can be identified reliably using scRRBS data
Cons:
- Lower transcriptome coverage than scMT-seq1
- Results in 3'-biased transcriptome
- scTrio-Seq: Hou Y., Guo H., Cao C., Li X., Hu B., et al. (2016) Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Res
- 1. Hu Y., Huang K., An Q., Du G., Hu G., et al. (2016) Simultaneous profiling of transcriptome and DNA methylome from a single cell. Genome Biol 17: 88
- Bock C., Farlik M. and Sheffield N. C. Multi-Omics of Single Cells: Strategies and Applications. Trends Biotechnol. 2016;34:605-608
- Poirion O. B., Zhu X., Ching T. and Garmire L. Single-Cell Transcriptomics Bioinformatics and Computational Challenges. Frontiers in Genetics. 2016;7:163
- Cheow L. F., Courtois E. T., Tan Y., et al. Single-cell multimodal profiling reveals cellular epigenetic heterogeneity. Nat Methods. 2016;13:833-836
- Picelli S. Single-cell RNA-sequencing: The future of genome biology is now. RNA Biol. 2016;1-14
- Qian M., Wang D. C., Chen H. and Cheng Y. Detection of single cell heterogeneity in cancer. Semin Cell Dev Biol.