Advance your research

Educational resources to help you learn about NGS and start using the iSeq 100 System

NGS System Buyer’s Guide

Use this guide to help you evaluate important factors and choose the right next-generation sequencing instrument for your lab.

Download Guide
Sequencing Platform Comparison Tool

This interactive tool will help you compare sequencers and find the one that’s right for your lab or application.

Compare Platforms
Benchtop Sequencer Comparison Table

Compare the iSeq 100 System with other Illumina benchtop sequencers.

Learn More
Introduction to NGS

This detailed overview describes the basics of Illumina sequencing technology, key methods, and more.

Access PDF
Introduction to Sequencing by Synthesis

See Illumina sequencing by synthesis (SBS) technology in action and learn how it works.

View Video
NGS vs. Sanger Sequencing

Learn the key differences between NGS and Sanger sequencing and determine when NGS can be a more effective option.

Learn More
NGS vs. qPCR

Compare the benefits of NGS technology to qPCR and learn which one to choose.

Learn More
NGS Library Preparation

Find kits, methods, and automation solutions to help you prepare DNA or RNA libraries before sequencing.

Learn More
Sequencing Data Analysis

Our software simplifies sequencing data analysis, so you can focus on your research instead of setting up analysis workflows.

Learn More
Benefits of Targeted Resequencing

Learn how targeted NGS can help you gain greater insight in your studies and save time compared to traditional methods.

Download Guide
One-Channel SBS

See how sequencing on a semiconductor chip reduces instrument cost and simplifies NGS on the compact iSeq 100 System.

Learn More
Instrument Buying Options

Explore different buying options—including leasing, bundles, trade-in offers, and more—to find one that fits your needs.

Learn More
Grant Writing Assistance

This document offers specification information and other resources to help you justify funding for the iSeq 100 System.

Access PDF
Illumina Financial Solutions

Acquire the advanced technology you need now through fast, easy, and affordable financing and leasing programs.

Learn More
iSeq 100 System Manuals and Documentation

Find supporting documentation for the iSeq 100 System.

Learn More
Sequencing Online Training

Online Illumina sequencing courses are free, interactive, and available any time.

Find Training
Sequencing Coverage

Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.

Learn More
Quality Scores

Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.

Learn More
Sequencing Troubleshooting Tips

These short videos provide expert tips for common sequencing issues such as overclustering and inconsistent quantification.

Learn More
Switching from Ion Torrent™ Systems to Illumina NGS Platforms

New to Illumina technology? See step-by-step guidance to help you transition your studies.

View Guide
AmpliSeq for Illumina on the iSeq 100 System

Find workflow guidance from Illumina experts, including considerations for hotspot resequencing with AmpliSeq for Illumina Cancer Hotspot Panels.

View Guide
Nextera DNA Flex on the iSeq 100 System

View tips on transitioning to the Nextera DNA Flex Library Prep Kit, including considerations for viral and bacterial whole-genome sequencing.

View Guide
Transitioning from TruSeq Custom Amplicon

See our detailed guides designed to help you transfer projects from TruSeq Custom Amplicon to AmpliSeq for Illumina panels.

View Guide
Library Prep Kit Selector

Determine the best sequencing library preparation kit for your needs based on your project type, starting material, and method of interest.

Find the Right Kit
Gene Panel Finder

Identify sequencing panels that target your genes of interest.

Find the Right Panel
Product Selection and Experiment Planning Tools

Find interactive tools designed to help you choose the right products for your needs and plan your experiments.

Learn More

iSeq 100 Educational Resources