Order the iSeq 100 System
The iSeq 100 System makes next-generation sequencing easier and more affordable than ever. Labs of all sizes can now easily sequence small genomes, interrogate gene sets, analyze gene expression, and more. Advance your research with powerful, cost-effective sequencing.
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Required Products
iSeq 100 Reagents
iSeq 100 Reagents include a flow cell and the necessary sequencing reagents for running the iSeq 100 System.
PhiX Control v3
This ready-to-use control library is derived from the small, well-characterized PhiX genome.
Compare Library Prep Kits
You will need one library preparation kit to prepare DNA or RNA libraries before sequencing. Find the right kit for your sample type and application.
Instrument Qualification Services
While the iSeq 100 System is easy for users to install, Illumina scientists and engineers can provide additional support to help you get started. Our qualification services help labs meet compliance requirements by verifying correct instrument setup and operation.
Explore Qualification Services
Illumina Product Care
A comprehensive one-year warranty at our Basic Plan service level is included with every new Illumina instrument purchase. We also offer tiered Product Care service plans, so you can choose the right level of service for your lab.
View Product Support Plans
Frequently Purchased Together
Product Literature
Product Information Sheet | PDF < 1 MB
Illumina Proactive Service connectivity guide - iSeq 100
Brochure | PDF < 1 MB
Brochure | PDF < 1 MB
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF < 1 MB
16S Metagenomics Sequencing on the iSeq 100 System
Application Note | PDF 4 MB
QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 System
Application Note | PDF 2 MB
Sequencing Library QC on the iSeq System
Application Note | PDF < 1 MB
Pan-Viral Sequencing on Illumina Benchtop Systems Application Note
Application Note | HTML
Microbial Whole-Genome Sequencing App Note
Application Note | PDF 5 MB
Product Information Sheet | PDF < 1 MB
Brochure | PDF | 5 versions
Microbial whole-genome sequencing with the iSeq 100 System
Application Note | PDF < 1 MB
Accurate surveillance of healthcare-acquired infections
Technical Note | PDF < 1 MB
Technical Note | PDF | 11 versions
Data Sheet | PDF | 7 versions
Data Sheet | PDF | 5 versions
Manuals and Support Information
iSeq 100 Sequencing System Guide Documentation
iSeq 100 Sequencing System Performance Qualification Documentation
iSeq 100 Sequencing System Setup Poster Documentation
RFID Reader Compliance Guide Documentation
iSeq 100 Sequencing System Installation Qualification and Operational Qualification Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
AmpliSeq for Illumina Cancer Hotspot Panel v2
Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
TruSeq Small RNA Library Preparation Kits
These kits provide a simple, cost-effective solution for generating miRNA and small RNA sequencing libraries directly from total RNA, for any species.
AmpliSeq for Illumina Focus Panel
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
Nextera XT DNA Library Preparation Kit
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
iSeq 100 Reagents
iSeq 100 Reagents feature a ready-to-use cartridge and one-channel SBS technology for simple, low-cost sequencing.