|Run Time||9–17.5 hrs|
|Instrument Dimensions||42.5 cm × 30.5 cm × 33 cm (16.8 in × 12 in × 13 in)|
|Weight||15.9 kg (35 lb)|
|Read Length||Total Time||Output|
|1 × 36 bp||9 hrs||144 Mb|
|1 × 50 bp||9 hrs||200 Mb|
|1 × 75 bp||10 hrs||300 Mb|
|2 × 75 bp||13 hrs||600 Mb|
|2 × 150 bp||17.5 hrs||1.2 Gb|
*Install specifications are based on Illumina PhiX control library at supported cluster densities (174–200 k/mm2 clusters passing filter). Total times include cluster generation, sequencing, and base calling with quality scores on an iSeq 100 System.
|Single Reads||4 million|
|Paired-End Reads||8 million|
|> 85% of bases higher than Q30 at 1 × 36 bp|
|> 85% of bases higher than Q30 at 1 × 50 bp|
|> 80% of bases higher than Q30 at 1 × 75 bp|
|> 80% of bases higher than Q30 at 2 × 75 bp|
|> 80% of bases higher than Q30 at 2 × 150 bp|
*The percentage of bases > Q30 is averaged across the entire run.
For more information about sequencing specifications and what they mean, view the following educational resources.
Sequencing quality scores are a measure of the uncertainty of base calls, or the probability of a base call being wrong.Learn More
Sequencing coverage requirements vary by application. Find out how to estimate and achieve your desired NGS coverage level.Learn More
Paired-end runs sequence both DNA ends, for easier analysis of rearrangements, novel transcripts, and more. Single-end runs offer an economical alternative.Learn More
The iSeq 100 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
The iSeq 100 System combines Illumina SBS with complementary metal-oxide-semiconductor (CMOS) technology to deliver one-channel SBS chemistry. One-channel SBS uses the same base-calling architecture as two-channel SBS. Two images are taken of each cluster during each sequencing cycle, and a simple binary “on/off” pattern determines the base that is called.Read Technical Note