Specifications for the iSeq 100 System

Lightweight, compact instrument makes sequencing simple while maintaining high data quality

iSeq 100 Specifications

Run Time 9–17.5 hrs
Instrument Dimensions 42.5 cm × 30.5 cm × 33 cm (16.8 in × 12 in × 13 in)
Weight 15.9 kg (35 lb)
Read Length Total Time Output
1 × 36 bp 9 hrs 144 Mb
1 × 50 bp 9 hrs 200 Mb
1 × 75 bp 10 hrs 300 Mb
2 × 75 bp 13 hrs 600 Mb
2 × 150 bp 17.5 hrs 1.2 Gb

*Install specifications are based on Illumina PhiX control library at supported cluster densities (174–200 k/mm2 clusters passing filter). Total times include cluster generation, sequencing, and base calling with quality scores on an iSeq 100 System.

iSeq 100 i1 Reagents
Single Reads 4 million
Paired-End Reads 8 million
iSeq 100 i1 Reagents
> 85% of bases higher than Q30 at 1 × 36 bp
> 85% of bases higher than Q30 at 1 × 50 bp
> 80% of bases higher than Q30 at 1 × 75 bp
> 80% of bases higher than Q30 at 2 × 75 bp
> 80% of bases higher than Q30 at 2 × 150 bp

*The percentage of bases > Q30 is averaged across the entire run.

iSeq 100 System Specification Sheet

System Specification Sheet

The smallest Illumina sequencing system delivers fast and efficient low-throughput sequencing for virtually any lab.

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Sequencing Quality Scores

Sequencing quality scores are a measure of the uncertainty of base calls, or the probability of a base call being wrong.

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Sequencing Coverage

Sequencing coverage requirements vary by application. Find out how to estimate and achieve your desired NGS coverage level.

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Paired-End vs. Single-Read Sequencing

Paired-end runs sequence both DNA ends, for easier analysis of rearrangements, novel transcripts, and more. Single-end runs offer an economical alternative.

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Sequencing by Synthesis (SBS) Technology

The iSeq 100 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.

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One-Channel SBS Technology

The iSeq 100 System combines Illumina SBS with complementary metal-oxide-semiconductor (CMOS) technology to deliver one-channel SBS chemistry. One-channel SBS uses the same base-calling architecture as two-channel SBS. Two images are taken of each cluster during each sequencing cycle, and a simple binary “on/off” pattern determines the base that is called.

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