AmpliSeq for Illumina BRCA Panel

Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.Read More...
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AmpliSeq™ BRCA Panel for Illumina®



Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®



AmpliSeq™ Library PLUS (96 Reactions) for Illumina®



AmpliSeq™ Library PLUS (384 Reactions) for Illumina®



Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)


Accessory Products

AmpliSeq™ for Illumina® Direct FFPE DNA



AmpliSeq™ Library Equalizer for Illumina®



Product Highlights

The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. Key features include:

Relevant Gene Content
  • Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis

The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina


Project Recommendations

Instrument Recommended Number of Samples Read Length
iSeq 100 System 12 samples per run (assumes minimum coverage of 500×)
96 samples per run (assumes minimum coverage of 50x)
2 × 150 bp
2 × 150 bp
MiniSeq System Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80
Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96
2 × 150 bp
2 × 150 bp

Product Comparison

AmpliSeq for Illumina BRCA Panel TruSight Cancer
Assay Time 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 1.5 days
Cancer Type Solid Tumor Pan-Cancer
Content Specifications Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers.
Description Germline and somatic analysis studies of BRCA1 and BRCA2 . Germline mutation detection research for common and rare cancers.
Hands-On Time < 1.5 hours 5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 50 ng DNA
Method Amplicon Sequencing , Targeted DNA Sequencing Target Enrichment , Target Enrichment, Targeted DNA Sequencing
Multiplexing 96 dual index combinations Up to 96-plex
Specialized Sample Types FFPE Tissue Not FFPE-Compatible
Species Category Human Human
Variant Class Germline Variants, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants Germline Variants

Method-Specific Workflow Example


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