A broad range of applications—all on one platform

Flexibility for virtually any genome, sequencing method, and scale of project

NovaSeq Applications & Methods

Whole-genome sequencing (WGS) is the most comprehensive method for analyzing the genome. Rapidly dropping sequencing costs and the ability to produce large volumes of data make WGS a powerful tool for genomics research of any species, including human, livestock, plants, and disease-related microbes.

Whole-exome sequencing (WES) can efficiently identify coding variants across a wide range of applications, including population genetics, genetic disease, and cancer studies.

Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. Sequence up to 132 exomes per NovaSeq run using dual S2 flow cells.

Total RNA sequencing (RNA-Seq) allows researchers to detect both known and novel features in a single assay. This method enables the detection of transcript isoforms, gene fusions, single nucleotide variants, allele-specific gene expression and other features without the limitation of prior knowledge.

Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. Sequence up to 132 transcriptomes per NovaSeq run using dual S2 flow cells.

* Run time based on dual S2 flow cells run on a NovaSeq 6000 System. Run times on the NovaSeq 5000 System may be slightly longer.

Coding Transcriptome Analysis

Detect both known and novel features of the coding transcriptome using sequence-specific capture of RNA coding regions.

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Targeted DNA Sequencing

Targeted resequencing focuses time, expenses, and analysis on sequencing only a subset of genes or genome regions of research interest.

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Methylation Sequencing

Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.

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Gene Expression Profiling with mRNA-Seq

mRNA sequencing (mRNA-Seq) detects known and novel transcripts and measures transcript abundance for accurate, comprehensive expression profiling.

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De Novo Sequencing

De novo sequencing refers to sequencing a novel genome with no reference sequence available. NGS enables fast, accurate characterization of any species.

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DNA-Protein Interaction Analysis with ChIP-Seq

Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.

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Population Studies

Robust, secure, and scalable platforms to aggregate and interpret large-scale genomic data for population sequencing applications.

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Comprehensively sample genes in organisms present in a complex sample to evaluate bacterial diversity and detect unculturable microorganisms.

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Illumina NGS Methods Guide

Illumina NGS Methods Guide

Access the information you need—from genome, transcriptome, or epigenome library preparation to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.

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