The S4 200 cycle kit is now available for NovaSeq Reagent Kits, providing additional flexibility to configure output and run time to meet your study needs. Select the S4 200 cycle kit option below.
|NovaSeq 6000 System|
|Flow Cell Type||SP||S1||S2||S4|
|Output Per Flow Cell|
|2 × 50 bp||65–80 Gb||134–167 Gb||333–417 Gb||N/A ‡|
|2 × 100 bp||N/A||266–333 Gb||667–833 Gb||1600–2000 Gb|
|2 × 150 bp||200–250 Gb||400–500 Gb||1000–1250 Gb||2400–3000 Gb|
|2 x 250 bp||325-400 Gb||N/A ‡||N/A ‡||N/A ‡|
|Clusters Passing Filter|
|Single Reads||650–800 million||1.3–1.6 billion||3.3–4.1 billion||8–10 billion|
|Paired-end Reads||1.3–1.6 billion||2.6–3.2 billion||6.6–8.2 billion||16–20 billion|
|Run Time §|
|2 × 50 bp||~13 hr||~13 hr||~16 hr||N/A|
|2 × 100 bp||N/A||~19 hr||~25 hr||~36 hr|
|2 × 150 bp||~25 hr||~25 hr||~36 hr||~44 hr|
|2 x 250 bp||~38 hr||~N/A||~N/A||~N/A|
* Output and read number specifications based on a single flow cell
using Illumina PhiX control library at supported cluster densities.
The NovaSeq 6000 System can run 1 or 2 flow cells simultaneously.
§ Run time includes cluster generation, sequencing, and base calling. Run times are based on running 2 flow cells of the same type; starting two different flow cells will impact run time.
Specifications based on Illumina PhiX control library at supported cluster densities.
‡ N/A: not applicable