NovaSeq 6000 Reagent Kits

Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.

Overview

NovaSeq 6000 Reagent Kits enable tunable output and proven performance for a broad range of applications on the NovaSeq 6000 system. These kits offer:

  • Ready-to-use reagent cartridges for streamlined operation 

  • Various read lengths to easily adjust output and sample throughput 

  • Multiple flow cell configurations to support an extensive output range

Increase lab efficiency

Improvements to the NovaSeq 6000 Reagent Kits include an enhanced reagent chemistry to streamline the workflow and accommodate more complex indexing strategies, an extended reagent shelf life of six months to provide project planning flexibility, and radio-frequency identification (RFID) to ensure reagent traceability and compatibility.

Flow cell configurations

SP and S1 provide the fastest run times and are ideal for smaller batch sizes or in situations where rapid turnaround time is required.

S2 offers a quick, powerful, and cost-effective option for high-throughput applications.

S4 delivers high throughput while enabling cost-effective sequencing across a range of applications and depth of coverage.

Additional benefits

NovaSeq 6000 Reagent Kits v1.5 also provide an improved Q30 quality score compared to the v1.0 reagents, support for unique molecular identifiers (UMIs), and support for whole-genome sequencing with Illumina DNA PCR-Free Library Prep. 


Specifications


Required products

No accessories are required, but additional library tubes for the NovaSeq 6000 Reagent Kits are available to order separately. 

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Documentation

Product literature

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v1.5 reagents (13)

NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles)

20028312

Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)

20028313

Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S4 Reagent Kit v1.5 (35 cycles)

20044417

Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 35-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S2 Reagent Kit v1.5 (300 cycles)

20028314

Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)

20028315

Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles)

20028316

Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 100-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S1 Reagent Kit v1.5 (300 cycles)

20028317

Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)

20028318

Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 S1 Reagent Kit v1.5 (100 cycles)

20028319

Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 100-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles)

20028402

Includes one SP flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 500-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 SP Reagent Kit v1.5 (300 cycles)

20028400

Includes one SP flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)

20040719

Includes one SP flow cell on,e buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.

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NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles)

20028401

Includes one SP flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 100-cycle run on the NovaSeq 6000 System.

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Accessory products (1)

NovaSeq Library Tubes Accessory Pack (24 tubes)

20014015

Includes 24 NovaSeq 6000 Library Tubes.

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Selection summary

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FAQs

NovaSeq 6000 Reagent Kits support both single-read and paired-end sequencing. During run setup, a run can be configured to generate single-read or paired-end data.

Yes. For NovaSeq reagents v1.5, follow the NovaSeq Series Custom Primers Guide.

The NovaSeq 6000 S4 flow cell provides 8–10B single reads and 16–20B paired-end reads.

The NovaSeq SP flow cell provides 650–800M single reads and 1.3–1.6B paired-end reads.

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