Now available: NovaSeq S4 Reagent Kits offering tunable output and additional flexibility on the NovaSeq 6000 System. Select the S4 option below when ordering.

NovaSeq Reagent Kits

Reagent kits for the NovaSeq 6000 System provide ready-to-use cartridge-based reagents for cluster generation and SBS. Read More...
Select Product(s)

NovaSeq 6000 S4 Reagent Kit (300 cycles)


NovaSeq 6000 S2 Reagent Kit (100 cycles)


NovaSeq 6000 S2 Reagent Kit (200 cycles)


NovaSeq 5000/6000 S2 Reagent Kit (300 cycles)


Accessory Products
What accessories do I need?

NovaSeq 6000 Library Tube Accessory Pack (24 tubes)


Product Highlights

NovaSeq 6000 Reagent Kits enable tremendous flexibility and unprecedented sequencing power for a broad range of applications on the NovaSeq 6000 System.

  • Ready-to-use reagent cartridges for streamlined operation
  • Radio-frequency identification (RFID) encoded consumables ensure reagent traceability and compatibility
  • Multiple flow cell and read length configurations support a wide range of applications
A New Standard in Ease of Use

The NovaSeq 6000 Reagent Kits contain three ready-to-use cartridges pre-filled with all the reagents needed for cluster generation and sequencing. The cartridges replace the multiple reagent bottles and tubes used on previous high-throughput systems, drastically simplifying and reducing run setup time. With features like RFID encoding for traceability and a snap-in design to minimize opportunities for errors, the NovaSeq Reagent Kits bring streamlined operation to high-throughput sequencing.


Supporting Data and Figures

NovaSeq 6000 System Flow Cell Specifications*
  NovaSeq 6000 System
Flow Cell Type S1 S2 S4
Output per Flow Cell  
2 × 50 bp 134 - 167 Gb 280 - 333 Gb N/A
2 × 100 bp 266 - 333 Gb 560 - 667 Gb N/A
2 × 150 bp 400 - 500 Gb 850 - 1000 Gb 2400 - 3000 Gb
Reads Passing Filter 1.3 - 1.6 billion 2.8 - 3.3 billion 8 - 10 billion
Run Time  
2 × 50 bp ~13 hr ~16 hr N/A
2 × 100 bp ~18 hr ~25 hr N/A
2 × 150 bp ~24 hr ~36 hr ~44 hr

* Output and read number specifications based on a single flow cell using Illumina PhiX control library at supported cluster densities. The NovaSeq 6000 System can run 1 or 2 flow cells simultaneously.
† NovaSeq S1 Reagent Kits are not currently available.
‡ Run time includes cluster generation, sequencing, and base calling. Run times are based on running 2 flow cells of the same type; starting two different flow cells will impact run time.


Product Literature

NovaSeq 6000 Sequencing System

Data Sheet | PDF < 1 MB

Manuals and Support Information

NovaSeq 6000 System Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All NovaSeq 6000 System Support 

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