Nextera Flex for Enrichment

A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.Read More...
Select Product(s)

Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)

20025524

Price
 
 

Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 16 samples (16, 1-plex enrichment reactions)

20025523

Price
 
 

Nextera DNA Flex Pre-Enrichment Library Prep Reagents (96 samples)

20025520

Price
 
 

Nextera DNA Flex Pre-Enrichment Library Prep Reagents (16 samples)

20025519

Price
 
 
Panel

Illumina Exome Panel - Enrichment Oligos only (8 or 12 Enrichment Reactions)

20020183

Price
 
 

TruSight Cancer – Enrichment Oligos only (4 or 8 Enrichment Reactions)

FC-121-0202

Price
 
 

TruSight One – Enrichment Oligos only (6 Enrichment Reactions)

20029227

Price
 
 

TruSight One Expanded – Enrichment Oligos only (6 Enrichment Reactions)

20029226

Price
 
 

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

Price
 
 

TruSeq Neurodegeneration - Enrichment Oligos only (8 Enrichment Reactions)

20029550

Price
 
 

Illumina Custom Enrichment Panel (8 Enrichment Reactions)

20025371


Index Adapters

IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

Price
 
 

IDT® for Illumina Nextera DNA Unique Dual Indexes Set B (96 Indexes, 96 Samples)

20027214

Price
 
 

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

Price
 
 

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

Price
 
 
Accessory Products

Flex Lysis Reagent Kit (96 reactions)

20018706

Price
 
 

Infinium FFPE QC Kit (384 reactions)

WG-321-1001

Price
 
 

Infinium MIDI Heatblock Insert

BD-60-601

Price
 
 

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Price
 
 

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Price
 
 

Product Highlights

Nextera Flex for Enrichment is the fastest and most flexible targeted sequencing solution for DNA in the Illumina library prep portfolio:

  • A time-saving solution that is 85% faster than standard Illumina library prep and enrichment
  • Integrated DNA extraction protocols for blood and saliva
  • Optimized performance using bead-based library prep chemistry for reliable results
Fast Library Prep and Enrichment Workflow

Nextera Flex for Enrichment uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time to ~6.5 hours. Go from DNA input and extraction to library normalization, enrichment, and post-enrichment amplification in just 6.5 hours. Total hands-on time is reduced to ~2 hours.

Integrated Sample Input

Nextera Flex for Enrichment supports a broad DNA input range (50–1000 ng) and multiple sample types, including blood, saliva, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue. Adding blood and saliva samples directly into the Nextera DNA Flex tagmentation reaction reduces the number of required quantification steps, saving time and money.

Optimized Performance

While accommodating various study requirements, the Nextera Flex for Enrichment solution delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible data across all Illumina sequencing systems.

Explore the Next Era in Library Prep

Discover the power that Nextera technology can bring to your lab through this interactive experience. Nextera library prep kit solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic
Introduction to Nextera DNA Flex Library Prep Kit

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
iSeq 100 System TruSight Cancer: 4 samples per run (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x)
2 x 100 bp

2 x 100 bp
MiniSeq System TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x)
2 x 100 bp

2 x 100 bp
MiSeq System TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x)
TruSight One (samples per run): v2 reagents: 1, v3 reagents: 2 (based on 120x mean targeted coverage; 95% target coverage at 20x)
2 x 100 bp

2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
NextSeq 550 System Exome (samples per run): mid output: 5, high output: 16 (based on 50x mean targeted coverage; 90% target coverage at 20X)
TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x)
TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x)
2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
2 x 100 bp (up to 2 x 150 bp is possible)
HiSeq 2500 System Exome (samples per run, single flow cell): rapid run: 12, high output with v4 reagents: 80 (based on 50x mean targeted coverage; 90% target coverage at 20X)
TruSight One (samples per run, single flow cell): rapid run: 27, high output with v4 reagents: 184 (based on 120x mean targeted coverage; 95% target coverage at 20x)
TruSight One Expanded (samples per run, single flow cell): rapid run: 18, high output with v4 reagents: 121 (based on 130x mean targeted coverage; 97% target coverage at 20x)
2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
2 x 100 bp
HiSeq 4000 System TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) 2 x 100 bp
NovaSeq 6000 System Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) 2 x 100 bp

Method-Specific Workflow Example

 

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Nextera DNA Flex Library Prep Kit

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.