NGS library prep methods

An extensive collection of published techniques for preparing sequencing libraries

Sequencing Library Preparation Methods

Use this interactive tool to explore experimental NGS library prep methods compiled from the scientific literature. The tool includes a subset of methods from the sequencing methods review articles and posters below. New methods will be added as they become available.

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Sequencing Method Explorer

View illustrated schematics of DNA, RNA, and single-cell library preparation techniques that are compatible with SBS technology. Also see brief diagrams of Illumina library preparation kit protocols.

For All You Seq—DNA

This poster includes NGS methods for analyzing:

  • DNA variants
  • Low levels of DNA
  • Epigenetics
  • DNA–protein interactions
  • Protein–protein interactions
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For All You Seq—RNA

This poster includes a comprehensive set of NGS methods for analyzing:

  • RNA transcription
  • RNA–protein interactions
  • Low levels of RNA
  • RNA modifications
  • RNA structure
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For All You Seq—Single-Cell

This poster includes a comprehensive set of NGS-based single-cell analysis methods for:

  • Low-level RNA detection
  • Low-level DNA detection
  • Integrated DNA and RNA analysis
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Sequencing Methods Posters
DNA and RNA Methods Reviews

Compare a Broad Range of Methods

Explore the world's largest collection of NGS library preparation techniques. These methods, compiled from the scientific literature, demonstrate the wide range of scientific questions you can address with Illumina sequencing by synthesis (SBS) technology.

These comprehensive review articles describe the various NGS methods, with pros and cons, publication summaries, and references.

Access DNA Methods ReviewAccess RNA Methods Review

PAIR uses PNAs to capture RBPs in vivo. , The PNAs are coupled to a cell membrane-penetrating peptide (CPP) to deliver PNAs efficiently into living cells, as well as a photoactivatable compound, p-benzoylphenylalanine (Bpa). The cells are illuminated with UV light, activating the Bpa on the PNA to form covalent bonds with the RBP. Next, the cells are lysed, and the RNA complexes are captured on magnetic beads. The proteins can be reverse-crosslinked and visualized by denaturing gel electrophoresis, while the RNA strands are sequenced.

Pros
  • Identifies RNA-protein interactions in vivo
  • Commercial PNAs can be purchased, eliminating the need for PNA design
Cons:
  • Custom PNA design can cause problems with low protein yield
  • Not yet adopted widely by the scientific community

Learn more about the advantages of sequencing by synthesis, how it works, and the many ways you can leverage sequencing technology to help answer your research questions.

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Methods Guide

All the information you need, from BeadChips to library preparation to sequencer selection and analysis. Select the best tools for your lab.

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