Use this interactive tool to explore experimental NGS library prep methods compiled from the scientific literature. The tool includes a subset of methods from the sequencing methods review articles and posters below. New methods will be added as they become available.Find a Method
View illustrated schematics of DNA, RNA, and single-cell library preparation techniques that are compatible with SBS technology. Also see brief diagrams of Illumina library preparation kit protocols.
This poster includes NGS methods for analyzing:
This poster includes a comprehensive set of NGS methods for analyzing:
This poster includes a comprehensive set of NGS-based single-cell analysis methods for:
Explore the world's largest collection of NGS library preparation techniques. These methods, compiled from the scientific literature, demonstrate the wide range of scientific questions you can address with Illumina sequencing by synthesis (SBS) technology.
These comprehensive review articles describe the various NGS methods, with pros and cons, publication summaries, and references.Access DNA Methods ReviewAccess RNA Methods Review
PAIR uses PNAs to capture RBPs in vivo. , The PNAs are coupled to a cell membrane-penetrating peptide (CPP) to deliver PNAs efficiently into living cells, as well as a photoactivatable compound, p-benzoylphenylalanine (Bpa). The cells are illuminated with UV light, activating the Bpa on the PNA to form covalent bonds with the RBP. Next, the cells are lysed, and the RNA complexes are captured on magnetic beads. The proteins can be reverse-crosslinked and visualized by denaturing gel electrophoresis, while the RNA strands are sequenced.