In May 2018, Illumina introduced the NextSeq™ 500/550 v2.5 sequencing reagent kits. With the availability of these new kits, the TruSight Cardio Sequencing Kit for NextSeq v2 will be obsolesced and upgrading to TruSight Cardio Sequencing Kit for NextSeq v2.5.
The last order date for v2 will be December 31, 2019 and the last ship date will be January 15, 2020. The library prep components of the kit have not changed. Please contact your local sales representative for additional information on this change.
TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)
FC-141-1010
Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)
20025524
The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiSeq System | 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
| NextSeq 550 System | 48 samples per run (mid output; based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
| TruSight Cardio Sequencing Kit | TruSight One Sequencing Panels | TruSight Inherited Disease | |
|---|---|---|---|
| Content Specifications | 575 Kb genomic content (174 genes) | TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
2.25 Mb genomic content (552 genes) |
| Description | Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions. | Fixed research panels targeting exonic regions that harbor disease-causing variants. | Fixed research panel focused on severe pediatric onset disorders. |
| Input Quantity | 50 ng DNA | 50 ng DNA | 50 ng DNA |
| Method | Target Enrichment , Target Enrichment, Targeted DNA Sequencing | Target Enrichment , Target Enrichment, Targeted DNA Sequencing | Target Enrichment , Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex | Up to 96-plex |
| Specialized Sample Types | Not FFPE-Compatible | Not FFPE-Compatible | Not FFPE-Compatible |
| Species Category | Human | Human | Human |
Illumina VariantStudio Data Analysis Software
Data Sheet | PDF2 MB
TruSight Cardio Sequencing Kit
Data Sheet | PDF< 1 MB
TruSight Cardio Sequencing Kit Gene List
product_file | EXCEL< 1 MB
TruSight Cardio Sequencing Kit Checklist Documentation
TruSight Cardio Sequencing Kit Consumables & Equipment Documentation
TruSight Cardio Sequencing Kit Support Documentation
TruSight Cardio Sequencing Kit Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
TruSight Inherited Disease is a targeted sequencing research panel that focuses on 552 genes associated with severe, recessive pediatric onset diseases.
* Data on file. Illumina, Inc. 2015.