TruSight Cardio enrichment oligos are now available separately for use with Illumina DNA Prep with Enrichment. The TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (FC-141-1010) and TruSight Cardio Sequencing Kits for NextSeq (FC-141-1011 and 20035190) have been discontinued.
|Instrument||Recommended Number of Samples||Read Length|
|MiSeq System||12 samples per run with v2 reagents (based on 300x mean coverage of targeted content)||2 × 150 bp (max recommended)|
|NextSeq 550 System||48 samples per run (mid output; based on 300x mean coverage of targeted content)||2 × 150 bp (max recommended)|
|TruSight Cardio Sequencing Kit||TruSight One Sequencing Panels||TruSight Inherited Disease|
|Content Specifications||575 Kb genomic content (174 genes)||TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
|2.25 Mb genomic content (552 genes)|
|Description||Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions.||Fixed research panels targeting exonic regions that harbor disease-causing variants.||Fixed research panel focused on severe pediatric onset disorders.|
|Input Quantity||50 ng DNA||50 ng DNA||50 ng DNA|
|Method||Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Target Enrichment , Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 96-plex|
|Specialized Sample Types||Not FFPE-Compatible||Not FFPE-Compatible||Not FFPE-Compatible|
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
* Data on file. Illumina, Inc. 2015.