TruSight Cardio enrichment oligos are now available separately for use with Illumina DNA Prep with Enrichment. The TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (FC-141-1010) and TruSight Cardio Sequencing Kits for NextSeq (FC-141-1011 and 20035190) have been discontinued.
The TruSight Cardio Panel uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1
Download this reference guide to understand which kit quantities you should purchase to support your research.
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| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiSeq System | 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
| NextSeq 550 System | 96 samples per run (mid output; based on 300x mean coverage of targeted content) | 2 × 150 bp (max recommended) |
| TruSight Cardio Sequencing Panel | TruSight One Sequencing Panels | |
|---|---|---|
| Content Specifications | 575 Kb genomic content (174 genes) | TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
| Description | Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions. | Fixed research panels targeting exonic regions that harbor disease-causing variants. |
| Input Quantity | 50 ng DNA | 50 ng DNA |
| Method | Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex |
| Specialized Sample Types | Not FFPE-Compatible | Not FFPE-Compatible |
| Species Category | Human | Human |
TruSight Cardio Sequencing Kit Gene List
product_file | EXCEL < 1 MB
TruSight Fixed Panel Combo Order Quantities
Product Information Sheet | PDF < 1 MB
TruSight Cardio Sequencing Panel
Data Sheet | PDF < 1 MB
TruSight Cardio Sequencing Kit Consumables & Equipment Documentation
TruSight Cardio Sequencing Kit Reference Guide Documentation
TruSight Cardio Sequencing Kit Checklist Documentation
TruSight Cardio Sequencing Kit Support Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
Assay targeting multiple variant types, including microsatellite instability (MSI) and tumor mutational burden (TMB).
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 System flow cell, without additional instrumentation.
These expert-defined sequencing research panels target 94 genes and 284 SNPs associated with a predisposition towards various cancers.
* Data on file. Illumina, Inc. 2015.