The TSO 500 ctDNA v2 is now available with improved sensitivity using lower cfDNA input, a faster turnaround time, and a more streamlined workflow.
TruSight Oncology 500 ctDNA v2
Enable CGP with a pan-cancer panel capable of detecting key IO gene signatures (TMB, MSI) plus all main variant classes from ctDNA in blood plasma.
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Select Product(s)
Library Prep
TruSight Oncology 500 ctDNA v2 plus Velsera Interpretation Report, for Use with NovaSeq 6000 S2 Flow Cell (24 samples)
20105907
Price
TruSight Oncology 500 ctDNA v2 plus Velsera Interpretation Report, for Use with NovaSeq 6000 S4 Flow Cell (24 samples)
20105908
Price
TruSight Oncology 500 ctDNA Kit plus Velsera Interpretation Report (16 indexes, 48 Samples)
20043410
Price
TruSight Oncology 500 ctDNA Kit for use with NovaSeq 6000 S2 plus Velsera interpretation report (16 indexes, 48 samples)
20102000
Price
TruSight Oncology 500 ctDNA Kit for use with NovaSeq 6000 S4 plus Velsera interpretation report (16 indexes, 48 samples)
20102001
Price
Index Adapters
Reagents
Software and Informatics Options
Illumina Connected Insights-Annual Subscription
20090137
Illumina Connected Insights‒Oncology Genome Equivalent Sample-VCF
20090138
Illumina Connected Insights‒Training and Onboarding
20092376
Informatics Professional Services
20071787
Total:
Product Highlights
TruSight Oncology 500 ctDNA v2 is a pan-cancer NGS assay that enables in-house comprehensive genomic profiling (CGP) of circulating tumor DNA (ctDNA) in blood plasma for research.
- Leverage minimally invasive blood samples as a complement or alternative to tissue biopsy
- Target 523 genes to assess DNA variants across major variant classes (SNV, MNV, indels, CNV, and gene rearrangements), plus key immuno-oncology gene signatures (TMB, MSI)
- Achieve ultra-sensitive detection from minimal cfDNA input
- Get final reports in <4 days from cfDNA with a streamlined workflow
Compatibility on the NovaSeq 6000 with NovaSeq 6000Dx (in RUO mode) and NovaSeq X coming in 2024!
Low-level biomarker detection
Achieve sensitive and accurate detection (0.2% VAF for SNVs) with low inputs (20 ng) using UMI-based hybrid-capture library prep and deep sequencing.
Integrated bioinformatics
DRAGEN secondary analysis powers a rapid variant calling algorithm on-premises or in the cloud with Illumina Connected Analytics. Access insights using integrated reporting with Illumina Connected Insights* or Velsera.
Streamlined workflow
Advanced chemistry and extensive workflow optimization enable a single-day library prep plus enrichment for a total turnaround time of <4 days. Automation-friendly kits and methods enable increased efficiencies.†
*Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
†Automation-friendly kits available in Q1 2024
TruSight Oncology ctDNA 500 v2
Illumina improved the analysis of circulating tumors with TruSight Oncology ctDNA 500 v2. Learn about new features and benefits in the video.
Enable CGP with a comprehensive portfolio
Maximize chances of identifying an actionable alteration with the TruSight Oncology 500 product line.
TruSight Oncology 500 ctDNA v2
Analyze circulating tumor DNA (ctDNA) in blood plasma with similar DNA panel content as TruSight Oncology 500.
TruSight Oncology 500
Assess relevant DNA and RNA cancer biomarkers from FFPE tumor tissue, now including HRD through an accessory kit to assess the Genomic Instability Score (GIS)†
TruSight Oncology 500 High-Throughput Assay
Batch up to 192 samples at a time while using the same panel content and tissue input type as TruSight Oncology 500.
†HRD is only available with the addition of the TruSight Oncology 500 HRD kit to the TruSight Oncology 500 kit. Not available in Japan.
TSO 500 portfolio brochure
Interested in learning more about the TSO 500 portfolio of products?
Download the overview brochureHave questions about our products? Connect with an Illumina representative.
Frequently Purchased Together
Specifications
Project Recommendations
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NovaSeq 6000 System | 24 samples per run (S4 flow cell), 800M paired-end reads, 35,000x coverage | 2 × 150 bp |
| NovaSeq 6000 System | 8 samples per run (S2 flow cell), 800M paired-end reads, 35,000x coverage | 2 × 150 bp |
Product Comparison
| TruSight Oncology 500 ctDNA v2 | TruSight Oncology 500 | TruSight Oncology 500 High-Throughput | |
|---|---|---|---|
| Cancer Type | Pan-Cancer, Solid Tumor | Pan-Cancer | Pan-Cancer |
| Method | Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing |
| Nucleic Acid Type | DNA | RNA, DNA | RNA, DNA |
| Specialized Sample Types | Blood, Circulating Tumor DNA | FFPE Tissue | FFPE Tissue |
| Species Category | Human | Human | Human |
| System Compatibility | NovaSeq 6000 | NextSeq 500, NextSeq 550, NextSeq 550Dx in Research Mode | NovaSeq 6000, NovaSeq 6000Dx in Research Mode |
| Technology | Sequencing | Sequencing | Sequencing |
| Variant Class | Copy Number Variants (CNVs), Insertions-Deletions (indels), Single Nucleotide Variants (SNVs) | Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants | Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants |
* Based on Pierian clinical knowledgebase, as of February 2023.
* NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.
* NovaSeq X and NovaSeq 6000 Dx compatibility coming in 2024
Case Studies
Illumina collaborates with National Cancer Center Japan to address a leading cause of death in Asia
Learn about this joint effort to accelerate the development of personalized treatments based on genomic information.
Learn moreSmall nucleotide variant detection at low VAF
Synthetic control samples with known VAF for each single nucleotide variant were diluted to values ranging from 0.20%–0.50% VAF and analyzed by TruSight Oncology 500 ctDNA v2
Indel detection at low VAF
Synthetic control samples with known VAF for each insertion or deletion were diluted to values ranging from 0.20%–0.50% VAF and analyzed by TruSight Oncology 500 ctDNA v2.
High TMB correlation compared to Tumor-Normal calling
Tumor-only workflow of TSO 500 ctDNA v2 utilizing advanced bioinformatics for germline and CH variant filtering produces highly concordant bTMB compared to a tumor-normal workflow.
Sensitive MSI detection for immune-oncology research
MSI was evaluated in 3 cell lines with known MSI-high status (samples 1–3) and detected down to 0.3% tumor fraction assessing up to ~2300 homopolymer sites.
Analytical Performance for CNV, LOD ≥1.3 fold for amplifications and ≤0.6 for deletions
*Lower relative MYC detection due to limit of detection approached faster due to fewer starting copies
Analytical Performance for gene rearrangements with detection at LOD (0.5%)
*VAF calculated by dividing total number of supporting reads by the higher depth of the two sides of the breakpoint, Illumina data on file 2023
Product Literature
Simplifying the process to identify applicable variants in oncology samples
Brochure | PDF 2 MB
Liquid Biopsy and NGS: Driving translational clinical research to the next level
Application Note | PDF < 1 MB
Technical Note | PDF | 2 versions
TruSight Oncology 500 ctDNA Gene List
product_file | EXCEL < 1 MB
Data analysis and reporting for the TruSight Oncology 500 portfolio
Product Information Sheet | PDF 1 MB
Data Sheet | PDF | 7 versions
TruSight Oncology 500 ctDNA v2
Data Sheet | PDF 1 MB
Manuals and Support Information
TruSight Oncology 500 ctDNA Support Documentation
DRAGEN TruSight Oncology 500 ctDNA Analysis Software v1.2 on ICA Documentation
TruSight Oncology 500 ctDNA Checklist Documentation
DRAGEN Analysis Workflows Product Documentation
DRAGEN TruSight Oncology 500 ctDNA Analysis Software v2.1.1 on ICA Documentation
TruSight Oncology 500 ctDNA Consumables & Equipment Documentation
TruSight Oncology 500 ctDNA Local App Documentation
TruSight Oncology 500 ctDNA v2 Product Documentation
DRAGEN TruSight Oncology 500 ctDNA Analysis Software v1.2 Documentation
DRAGEN TruSight Oncology 500 ctDNA Local Analysis Software v2.1 Documentation
DRAGEN TruSight Oncology 500 ctDNA v1.1 Local Analysis Software Documentation
NovaSeq Xp Workflow
The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 System flow cell, without additional instrumentation.
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with exceptional study flexibility.
NovaSeq 6000 Reagent Kits
Reagent kits for the NovaSeq 6000 system provide ready-to-use cartridge-based reagents for cluster generation and SBS.
TruSight Oncology 500 High-Throughput
Enabling comprehensive genomic profiling from FFPE samples, with added flexibility to batch up to 192 samples per flow cell.
Illumina Stranded mRNA Prep
A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA.
TruSight Oncology 500 ctDNA panel to be used across a number of Asian countries to develop personalized cancer treatments for Nasopharyngeal Carcinoma. View Press Release