Currently, the product is available for preorder only. TruSight Oncology 500 ctDNA is expected to be available for shipment in 2020.
Contact your Illumina representative to take advantage of an introductory promotional offer that is available for a limited time.

TruSight Oncology 500 ctDNA

Assay targeting multiple somatic variant types from plasma, including tumor mutational burden (TMB), and microsatellite instability (MSI). Read More...
Select Product(s)

TruSight Oncology 500 ctDNA, For Use with NovaSeq 6000 (S2 Reagent Kit) (16 indexes, 48 samples, 8 samples per run)

20039253

TruSight Oncology 500 ctDNA, For Use with NovaSeq 6000 (S4 Reagent Kit) (16 indexes, 48 samples, 24 samples per run)

20039254

Accessory Products

NovaSeq 6000 S2 Reagent Kit (300 cycles)

20012860

Price
 
 

NovaSeq 6000 S4 Reagent Kit (300 cycles)

20012866

Price
 
 

NovaSeq Xp 4-Lane Kit

20021665

Price
 
 

Product Highlights

An NGS-based assay that assesses multiple variant types in 523 cancer-related genes from cell-free DNA (cfDNA). Leveraging the power of the NovaSeq 6000 System for high depth of sequencing, unique molecular identifiers (UMIs), and the ultra-rapid DRAGEN Bio-IT Platform, provides high sensitivity and specificity to enable comprehensive genomic profiling from cfDNA.

Enable Comprehensive Genomic Profiling in Plasma

  • Same DNA content as TruSight Oncology 500 for detection of single nucleotide variants (SNVs), indels, CNVs, DNA fusions, TMB, and MSI

Achieve Rapid, Accurate Variant Detection

  • Highly sophisticated variant calling algorithm powered and accelerated by DRAGEN

Achieve Confidence in Results

  • Hybrid-capture chemistry, UMIs and high depth of coverage, and sophisticated error correction improve variant calling and reduce artifacts

Leverage the Power of the NovaSeq 6000 System

  • Enable cfDNA analysis with the required depth of sequencing for low-level variant detection in plasma

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NovaSeq 6000 System 24 samples per run (S4 flow cell), 800M paired-end reads, 35,000x coverage 2 × 150 bp
NovaSeq 6000 System 8 samples per run (S2 flow cell), 800M paired-end reads, 35,000x coverage 2 × 150 bp

Product Comparison

TruSight Oncology 500 ctDNA TruSight Oncology 500 TruSight Tumor 170
Assay Time 5 days from sample to variant report 3-4 days from sample to answer ~2 days (Library Prep)
Cancer Type Pan-Cancer Pan-Cancer Solid Tumor
Content Specifications Targeted selection of DNA from 523 genes of interest for a total of 1.94Mb panel size. Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size. Probes enrich for full coding sequences of 170 genes. Calls single nucleotide variants, small insertions, and deletions in 151 genes, amplifications in 59 genes, and fusions plus splice variants in 55 genes.
Hands-On Time ~10.5 hours ~10.5 hours ~10.5 hours
Input Quantity 30 ng cfDNA (8-10 ml of plasma) 40 ng DNA, 40 ng RNA (FFPE volume of .65 mm3 – 5 unstained slides) 40 ng DNA and/or RNA
Method Target Enrichment , Target Enrichment, Targeted DNA Sequencing Immunosequencing, Target Enrichment , Target Enrichment, Targeted DNA Sequencing , Targeted RNA Sequencing Target Enrichment , Target Enrichment, Targeted DNA Sequencing , Targeted RNA Sequencing
Nucleic Acid Type DNA RNA, DNA RNA, DNA
Specialized Sample Types Blood, Cell-Free DNA, Circulating Tumor DNA FFPE Tissue FFPE Tissue, Low-Input Samples
Species Category Human Human Human
System Compatibility NovaSeq 6000 NextSeq 500 , NextSeq 550 , NextSeq 550Dx in Research Mode HiSeq 2500 , NextSeq 500 , NextSeq 550
Technology Sequencing Sequencing Sequencing
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants

Method-Specific Workflow Example

 

Product Literature

TruSight Oncology 500 ctDNA

Data Sheet | PDF< 1 MB

Manuals and Support Information

A dedicated support section is not currently available for this product

Related Products

TruSight Oncology 500

Assay targeting multiple variant types, including tumor mutational burden (TMB) and microsatellite instability (MSI), even from low-quality samples.


TruSight Tumor 170

Comprehensive next-generation sequencing (NGS) assay that targets DNA and RNA variants from the same FFPE sample.


TruSight Oncology

TruSight Oncology is a set of NGS platform reagents for the detection of cancer variants, using an enrichment-based method to simultaneously analyze DNA and RNA. Gene content is not included.