|Instrument||Recommended Number of Samples||Read Length|
|MiSeq System||TruSight One: 3 samples per run with v3 reagents (based on 100x mean coverage of targeted content)||Up to 2 × 150 bp|
|NextSeq 550 System||TruSight One (samples per run): mid output: 12, high output: 36 (based
on 100x mean coverage of targeted content)
TruSight One Expanded: 24 samples per run (high output; based on 100x mean coverage of targeted content)
|Up to 2 × 150 bp
Up to 2 × 150 bp
|HiSeq 2500 System||TruSight One Expanded (samples per run): rapid run: 18, high output: 95 (based on 100x mean coverage of targeted content)||Up to 2 × 150 bp|
|NovaSeq 6000 System||TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content)||Up to 2 × 150 bp|
|TruSight One Sequencing Panels||TruSight Inherited Disease||TruSight Cardio Sequencing Kit|
|Content Specifications||TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
|2.25 Mb genomic content (552 genes)||575 Kb genomic content (174 genes)|
|Description||Fixed research panels targeting exonic regions that harbor disease-causing variants.||Fixed research panel focused on severe pediatric onset disorders.||Targeted sequencing research panel to identify somatic variants associated with 17 inherited cardiac conditions.|
|Input Quantity||50 ng DNA||50 ng DNA||50 ng DNA|
|Method||Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Target Enrichment , Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 96-plex|
|Specialized Sample Types||Not FFPE-Compatible||Not FFPE-Compatible||Not FFPE-Compatible|
Due to the larger size of the TruSight One Expanded Panel, Illumina recommends using a NextSeq, HiSeq, or NovaSeq system to achieve optimal sequencing coverage.
For the 36-sample TruSight One Sequencing Panel (Catalog No. FC-141-1007), the instrument-specific sequencing reagents must be purchased separately.