For use with the Genome Analyzer IIx and the Cluster Station, the TruSeq SR Cluster Kit v5-CS-GA provides reagents that bind samples to complementary adapter oligos for cluster amplification on single-read flow cells.
The TruSeq SR Cluster Kit v5-CS-GA provides master-mixed reagents for cluster generation using the Cluster Station.
DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.