ScriptSeq Complete libraries were constructed from 5 μg of B. subtilis (A) or E. coli (B) total RNA samples and analyzed for transcriptome content and coverage. All sequencing reads contain very high (98%+) coding and non-coding content. Ribosomal content was very low, minimizing wasted reads.
Cluster-ready ScriptSeq RNA-Seq libraries, virtually rRNA-free, are made in 1 day from as little as 100 ng of intact or partially degraded total RNA.
ScriptSeq Complete (Bacteria) removes ribosomal RNA from gram-positive, gram-negative, and mixed samples prior to library generation.
The low input versions of this kit (catalog numbers SCL6B and SCL24B) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.