ScriptSeq Complete Kit (Bacteria)

This kit removes ribosomal RNA from metagenomic samples (including Gram-positive and Gram-negative bacterial mixtures), and generates sequencing libraries. Read More...
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Scriptseq Complete (Bacteria) with Ribo-Zero Bacteria rRNA Removal (6 reactions)

BB1206

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ScriptSeq Complete Kit (Bacteria) 24 reactions

BB1224

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Accessory Products
What accessories do I need?

ScriptSeq Index PCR Primers (Set 1)

RSBC10948

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ScriptSeq Index PCR Primers (Set 2)

SSIP1202

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ScriptSeq Index PCR Primers (Set 3)

SSIP1203

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ScriptSeq Index PCR Primers (Set 4)

SSIP1204

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ScriptSeq Index PCR Primers (Sets 1-4)

SSIP1234

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Product Highlights

  • Removes ribosomal RNA (rRNA) from intact and partially degraded mixtures of Gram (+) and Gram (–) bacteria in a single pass
  • Creates a stranded Illumina sequencing library
  • Sequencing data contains complete transcriptome of coding and non-coding RNA species
  • Barcode/index-compatible: ScriptSeq index primers available separately for preparing barcoded libraries

Sequencing is an important discovery tool for metagenomics studies. The sequencing data from samples treated with ScriptSeq Complete (Bacteria) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

ScriptSeq Complete (Bacteria) offers the most informative sequencing results by removing unwanted rRNA prior to creation of a stranded sequencing library. ScriptSeq Complete (Bacteria) is formulated to efficiently remove rRNA from metagenomic samples that range from pure Gram (+) to pure Gram (-) and all combinations. When performing stranded analysis on samples prepared with ScriptSeq Complete (Bacteria), choose the FR=secondstrand option.

Related Information

Specifications

Supporting Data and Figures

 

rRNA Types Removed

Bacterial rRNA Removed
Gram-negative Gram-positive
23S 16S 5S 23S 16S 5S

ScriptSeq Complete (Bacteria) removes ribosomal RNA from gram-positive, gram-negative, and mixed samples prior to library generation.

Product Literature

Literature is not currently available for this product.

The low input versions of this kit (catalog numbers SCL6B and SCL24B) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.