NET-Seq
Break-Seq
Break-seq combines DSB labeling with NGS to map chromosome breaks with improved sensitivity and resolution. DNA trapped in agarose plugs is end-repaired and labeled with biotin. The agarose is then digested by beta-agarase, followed by dilution and fragmentation. The fragmented DNA is purified and end-repaired with unmodified dNTPs, followed by A-tailing. The fragments are purified on magnetic beads, PCR-amplified, purified, and used to prepare a sequencing library.
Pros:
- Simple protocol
Cons:
- Not replicated in other laboratories