nuc-seq/SNES

nuc-seq/SNES

Nuc-seq is a modified MDA protocol that takes advantage of the fact that a single cell in the G2–M stage of the cell cycle has four copies of the genome, which allows the cells to be isolated with a cell sorter and it also significantly increases the genome coverage of single cells. SNES, single nucleotide exome sequencing, is an additional variation that includes targeted selection and sequencing of the exomes.

Div-Seq is a variation, which combines nuc-seq with EdU-mediated labeling of proliferating cells.

Pros:

• nuc-seq: Improves physical coverage performance to more than 90% for single cell sequencing
• SNES: 95.94% exome coverage in single cells
• SNES: Detection efficiencies of 92.37% for SNVs in an isogenic population

Cons:

• nuc-seq: Cannot be applied to cells with low proliferation rates
• SNES: Limited to exomes

• nuc-seq: Wang Y., Waters J., Leung M. L., Unruh A., Roh W., et al. (2014) Clonal evolution in breast cancer revealed by single nucleus genome sequencing. Nature 512: 155-160
• SNES: Leung M. L., Wang Y., Waters J. and Navin N. E. (2015) SNES: single nucleus exome sequencing. Genome Biol 16: 55

1. Leung M. L., Wang Y., Kim C., Gao R., Jiang J., et al. (2016) Highly multiplexed targeted DNA sequencing from single nuclei. Nat Protoc 11: 214-235
2. Leung M. L., Wang Y., Waters J. and Navin N. E. (2015) SNES: single nucleus exome sequencing. Genome Biol 16: 55