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Exome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. With both rapid Run and High Output modes, HiSeq 2500 System is a flexible system that can sequence 5–75 exomes* per run.
*Assumes 100x coverage with 80% on target using 2 x 75 bp reads.
See how researchers are using exome sequencing on the HiSeq Series.
2.5 days/6 hours hands-on time
A cost-effective library preparation and exome enrichment solution with industry-leading accuracy.
5 days/30 minutes hands-on
Ready-to-load reagents for sequencing by synthesis on enabled HiSeq sequencing systems.
Reagents for paired-end cluster generation on the cBot cluster amplification system.
2–3 hours
Rapid alignment and variant detection for small, structural, and copy number variant calling, variant annotation, and enrichment metrics calculation.
Analyze both coding RNA and multiple forms of noncoding RNA with total RNA sequencing (RNA-Seq) for a comprehensive view of the transcriptome. With 4 billion reads per dual flow cell HiSeq run using the HiSeq v4 chemistry, the HiSeq 2500 System can sequence up to 80 transcriptomes* per run.
*Assumes 50 million reads per sample.
See how researchers are using total RNA-Seq on the HiSeq Series.
10.5 hours/4.5 hours hands-on
A clear, comprehensive view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis.
5 days/30 minutes hands-on
Ready-to-load reagents for sequencing by synthesis on enabled HiSeq sequencing systems.
Reagents for paired-end cluster generation on the cBot cluster amplification system.
~13 hours
Performs read mapping, FPKM estimation of reference genes and transcripts, novel transcripts assembly, variant calling, and optional fusion calling.
Quickly assess novel transcript isoforms and gene expression levels from RNA-Seq Alignment results.
mRNA sequencing (mRNA-Seq) detects known and novel transcripts and measures transcript abundance for accurate, comprehensive expression profiling.
Sequencing large complex genomes (human, plant, or animal) provides a base-by-base view of genomic alterations to inform studies of disease or population genomics.
Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.
Perform epigenetic studies with high coverage density and flexibility enabled by sequencing-based DNA methylation analysis.
Detect both known and novel features of the RNA exome using sequence-specific capture of RNA coding regions.
With a unique mix of high output, long reads, and paired-end sequencing, HiSeq is a powerful tool for de novo sequencing of species without a reference genome.
Perform metagenomic analyses by sequencing entire microbial communities to discover important taxonomic and functional information.
Genotyping by sequencing provides a low-cost genetic screening method to discover novel plant and animal SNPs and perform genotyping studies.
Small genome sequencing provides comprehensive analysis of microbial or viral genomes for public health, epidemiology, and disease studies.
Isolate and sequence small RNA species, such as microRNA, to study the role of noncoding RNA in gene silencing and posttranscriptional regulation.
Targeted gene sequencing panels contain defined probe sets focused on specific genes of interest. Both predesigned and custom panels are available.
A culture-free method to identify and compare bacteria from complex microbiomes or environments that are difficult to study.
Ribosome profiling enables systematic monitoring of cellular translation processes and prediction of protein abundance.
Targeted RNA sequencing (RNA-Seq) focuses on specific transcripts of interest, used to analyze gene expression and identify fusion genes.
Ultra-deep sequencing of PCR amplicons enables cost-effective analysis of up to hundreds of target genomic regions in one assay.
Next-generation sequencing offers base-by-base, genome-wide detection of chromosomal variation to complement array-based cytogenomic analysis.
Robust, secure, and scalable platforms to aggregate and interpret large-scale genomic data for population sequencing applications.
Assess the quality of a sequencing library before committing it to a full-scale run, or perform quality control applications for bioproduction studies.
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.
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