Exome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. With both rapid Run and High Output modes, HiSeq 2500 System is a flexible system that can sequence 5–75 exomes* per run.
*Assumes 100x coverage with 80% on target using 2 x 75 bp reads.
See how researchers are using exome sequencing on the HiSeq Series.
Analyze both coding RNA and multiple forms of noncoding RNA with total RNA sequencing (RNA-Seq) for a comprehensive view of the transcriptome. With 4 billion reads per dual flow cell HiSeq run using the HiSeq v4 chemistry, the HiSeq 2500 System can sequence up to 80 transcriptomes* per run.
*Assumes 50 million reads per sample.
Performs read mapping, FPKM estimation of reference genes and transcripts, novel transcripts assembly, variant calling, and optional fusion calling.
Quickly assess novel transcript isoforms and gene expression levels from RNA-Seq Alignment results.
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.Access Guide