|MiSeq Reagent Kit v2||MiSeq Reagent Kit v3|
|> 90% bases higher than Q30 at 1 × 36 bp||> 85% bases higher than Q30 at 2 × 75 bp|
|> 90% bases higher than Q30 at 2 × 25 bp||> 70% bases higher than Q30 at 2 × 300 bp|
|> 80% bases higher than Q30 at 2 × 150 bp|
|> 75% bases higher than Q30 at 2 × 250 bp|
† A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
The MiSeq System harnesses proven Illumina SBS technology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
Paired-end sequencing enables both ends of the DNA fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads. This is especially helpful across difficult-to-sequence, repetitive regions of the genome.