Patterned Flow Cell Technology

Breakthrough Boost in Sequencing Power

Our innovative patterned flow cell technology offers an exceptional level of throughput for diverse sequencing applications. Patterned flow cells use distinct nanowells for cluster generation to make more efficient use of the flow cell surface area. This advanced flow cell design contributes to increased data output, reduced costs, and faster run times.

Distinct, Ordered Nanowell Design

Patterned flow cells contain billions of nanowells at fixed locations across both surfaces of the flow cell (Figure 1). The structured organization provides even spacing of sequencing clusters to deliver significant advantages over non-patterned cluster generation.

  • Clusters can only form in the nanowells, making the flow cells less susceptible to overloading, and more tolerant to a broader range of library densities.
  • Precise nanowell positioning eliminates the need to map cluster sites, and saves hours on each sequencing run.
  • Higher cluster density leads to more usable data per flow cell, driving down the cost per gigabase (Gb) of the sequencing run.

Figure 1. Advanced Patterned Flow Cell Design Enables Maximum Throughput.
Patterned flow cells contain billions of nanowells at fixed locations, providing even cluster spacing and uniform feature size to deliver extremely high cluster density.

Prearranged Cluster Distribution on the Flow Cell

Patterned flow cells are produced using semiconductor manufacturing technology. Starting with a glass substrate, patterned nanowells are etched into the surface for optimal cluster spacing. Each nanowell contains DNA probes used to capture prepared DNA strands for amplification during cluster generation. The regions between the nanowells are devoid of DNA probes.

The process ensures that DNA clusters only form within the nanowells, providing even, consistent spacing between adjacent clusters and allowing accurate resolution of clusters during imaging. Maximal use of the flow cell surface leads to overall higher clustering.

Exclusion Amplification Chemistry

Our proprietary exclusion amplification clustering method further increases data output. Exclusion amplification allows simultaneous seeding (landing of the DNA strand in the nanowell) and amplification during cluster generation, which reduces the chances of multiple library fragments amplifying in a single cluster. This method maximizes the number of nanowells occupied by DNA clusters originating from a single DNA template, increasing the amount of usable data from each run.

Advanced Systems Featuring Patterned Flow Cell Technology

Patterned flow cell technology, pioneered on the HiSeq X Ten System, makes data-intensive applications more cost-effective, including the $1000 human genome. The HiSeq 3000/HiSeq 4000 Systems were the first Illumina sequencers to use patterned flow cells for diverse genomic applications.

The NovaSeq Series unites the latest high-performance imaging with the next generation of Illumina patterned flow cell technology. The updated NovaSeq flow cell design further reduces the spacing between nanowells, significantly increasing cluster density and data output.

 

See Patterned Flow Cells in Action

Patterned Flow Cell Technology VideoPatterned Flow Cell Technology

Check out this 3D video that shows how our innovative patterned flow cells work with our SBS technology.

Get Inside the Technology

Learn more about the innovative patterned flow cell technology and how it dramatically increases data throughput and run speed.
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