Intuitive Analysis of RNA-Seq Data
Once the domain of bioinformatics experts, RNA sequencing (RNA-Seq) data analysis is now more accessible than ever. Illumina offers push-button RNA-Seq software tools packaged in intuitive user interfaces designed for biologists.
These user-friendly tools support a broad range of next-generation sequencing (NGS) studies, from gene expression analysis to total RNA expression profiling and more.
Benefits of RNA-Seq Data Analysis with Illumina Tools
RNA-Seq data can be instantly and securely transferred, stored, and analyzed in BaseSpace Sequence Hub, the Illumina genomics cloud computing platform. In addition, the Illumina DRAGEN Bio-IT Platform provides accurate, ultra-rapid secondary analysis of RNA-Seq and other NGS data, in BaseSpace Sequence Hub or on-premise. Our BaseSpace RNA-Seq Apps are:
- Accessible to any researcher, regardless of bioinformatics experience
- An expert-preferred suite of RNA-Seq software tools, developed or optimized by Illumina or from a growing ecosystem of third-party app providers
- Designed to support common transcriptome studies, from gene expression quantification to detection of novel transcripts, coding single nucleotide polymorphisms (cSNPs), gene fusions, and more
- Suitable for human, mouse, and rat RNA-Seq analysis (certain apps also support additional species)
- Compatible with all Illumina sequencing systems
Learn more about:
RNA-Seq Data Analysis FAQs
Demystifying RNA sequencing data analysis with answers to common questions and concerns.
Key RNA-Seq Analysis Apps
Based on frequently cited RNA-Seq analysis pipelines, these apps support a broad range of transcriptome data analysis needs. Featured data analysis apps for common RNA-Seq methods are shown in the table below.
Core Lab Uses BaseSpace to Analyze mRNA-Seq Data
BRC-Seq uses BaseSpace Sequence Hub to analyze and share data (primarily mRNA-Seq data), and track samples.
| Method | Featured App |
|---|---|
| mRNA Sequencing | |
| Targeted RNA Sequencing | BaseSpace RNA Amplicon App |
| Small RNA Sequencing | BaseSpace Small RNA App |
SARS-CoV-2 NGS Data Toolkit
Illumina is making it simpler for researchers to detect and identify SARS-CoV-2 and contribute their findings to the community with an integrated software toolkit, free of charge.
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How to Analyze RNA-Seq Data with BaseSpace Apps
Walk through a typical BaseSpace Sequence Hub RNA sequencing data analysis workflow. Learn how to analyze your results, and view examples of the easily interpretable tables and graphs that the software generates.
Read Technical Note
Interpretation of RNA-Seq Data
After data analysis, results can be transferred easily to BaseSpace Correlation Engine for functional annotation, to understand the biological effects of gene expression changes. BaseSpace Correlation Engine contains data sets from thousands of public studies that inform biological interpretation. Connect differential gene expression data from RNA-Seq experiments with disease associations, or visualize correlated genes and microRNA targets.
Explore BaseSpace Correlation EngineSimplifying Genomic Data Mining
Learn how BaseSpace Correlation Engine facilitates genomic data interpretation. See examples of distinguished universities and pharmaceutical companies that have cited this tool in peer-reviewed publications.
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Additional Resources
A Tale of Two RNA Library Prep Kits
Comparative RNA-Seq data analysis from two TruSeq RNA kits revealed key differences in variant and fusion detection.