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Once the domain of bioinformatics experts, RNA sequencing (RNA-Seq) data analysis is now more accessible than ever. Illumina offers push-button RNA-Seq software tools packaged in an intuitive user interface designed for biologists.
These user-friendly tools support a broad range of next-generation sequencing (NGS) studies, from gene expression analysis to total RNA expression profiling and more.
RNA-Seq data can be instantly and securely transferred, stored, and analyzed in BaseSpace Sequence Hub, the Illumina genomics computing platform. BaseSpace Sequence Hub includes an expert-preferred suite of RNA-Seq software tools that were developed or optimized by Illumina. Our BaseSpace RNA-Seq Apps are:
Demystifying RNA sequencing data analysis with answers to common questions and concerns.
Based on frequently cited RNA-Seq analysis pipelines, these apps support a broad range of transcriptome data analysis needs. Illumina BaseSpace RNA-Seq Apps for common methods are shown in the table below.
View sample data and read customer feedback about how easy it is to use BaseSpace Apps for RNA-Seq.
| Method | Featured App |
|---|---|
| mRNA Sequencing | |
| Targeted RNA Sequencing | BaseSpace TruSeq Targeted RNA App |
| Small RNA Sequencing | BaseSpace Small RNA App |
Walk through a typical BaseSpace Sequence Hub RNA sequencing data analysis workflow. Learn how to analyze your results, and view examples of the easily interpretable tables and graphs that the software generates.
Read Technical Note
After data analysis, results can be transferred easily to BaseSpace Correlation Engine for functional annotation, to understand the biological effects of gene expression changes. BaseSpace Correlation Engine contains data sets from thousands of public studies that inform biological interpretation. Connect differential gene expression data from RNA-Seq experiments with disease associations, or visualize correlated genes and microRNA targets.
Explore BaseSpace Correlation EngineLearn how BaseSpace Correlation Engine facilitates genomic data interpretation. See examples of distinguished universities and pharmaceutical companies that have cited this tool in peer-reviewed publications.
The BRC-Seq core lab uses BaseSpace Sequence Hub to analyze and share mRNA-Seq and other data, and facilitate sample tracking.
Interpret and validate mutations that affect mRNA splicing and protein coding with the MutationForecaster App.
Access user guides and additional technical information about BaseSpace Apps.
Comparative RNA-Seq data analysis from two TruSeq RNA kits revealed key differences in variant and fusion detection.