Sequencing only the coding regions of the genome enables researchers to focus on the genes most likely to affect phenotype, and offers an accessible combination of turnaround time and price.
NextSeq 1000 and NextSeq 2000 System applications and methods
Flexibility to do more
Fuel a broad range of applications, from targeted panels to whole-genome sequencing and more
Key applications and methods
Library prep
Illumina DNA Prep with Enrichment + Illumina Exome Panel
A fast, integrated whole-exome enrichment and sequencing library preparation workflow for a wide range of applications.
Sequencing
NextSeq 1000/2000 XLEAP-SBS reagent kits
NextSeq 1000/2000 XLEAP-SBS reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Data analysis
Push-button alignment, followed by variant calling of Illumina exomes to rapidly identify disease-associated variants.
mRNA-Seq detects known and novel transcripts and measures transcript abundance for accurate, comprehensive expression profiling.
Library prep
A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA.
Sequencing
NextSeq 1000/2000 XLEAP-SBS reagent kits
NextSeq 1000/2000 XLEAP-SBS reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Data analysis
Perform alignment, quantification, and fusion detection.
Assess the individual contributions of single cells in complex tissues by profiling the transcriptome.
Library prep
10x Genomics Chromium Single Cell Gene Expression
This solution provides single-cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell.
Sequencing
NextSeq 1000/2000 XLEAP-SBS reagent kits
NextSeq 1000/2000 XLEAP-SBS reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Data analysis
DRAGEN Single-Cell RNA Pipeline
This integrated pipeline goes from base calls to a cell-by-gene-expression matrix with a single user touchpoint, compatible with a variety of third-party sample preparation kits. Available on the NextSeq 2000 System as well as on the DRAGEN Server.
Featured application resources
In this webinar, learn how the NextSeq 2000 system delivers the flexibility to support your research needs, including bulk RNA-Seq, target enrichment, single-cell RNA-Seq, and much more.
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Download this ebook to learn how Illumina benchtop sequencing systems are making NGS technology more accessible to laboratories worldwide. These systems provide the speed, power, and flexibility to make breakthroughs in microbiology, cancer research, and more. The MiSeq i100 Series or NextSeq 1000 and NextSeq 2000 Systems can help make your NGS research goals within reach.
In this tech note, learn how to get high-quality metagenomic data using 600 cycle kits on NextSeq 1000 and NextSeq 2000 Systems for longer reads and higher read depths to analyze metagenomic data from real-world samples.
The P3 and P4 flow cells on the NextSeq 2000 System are an excellent fit for multiomics studies. Use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single-application approaches.
Learn how Zymo Research advances microbiome research with the NextSeq 2000
Learn how Zymo Research advances microbiome research with the NextSeq 2000
Hear how the NextSeq 2000 System provides scientists with the precision, affordability, and accessibility to make their research a reality. With the help of this system, Zymo Research is delivering cost-effective and timely sequencing services and advancing microbiome research.
In this interview, Dr. Irina Iordanescu, the coordinator of the Regina Maria Genetic Center Laboratory, explains how NGS enhanced assays such as whole-exome sequencing and targeted sequencing panels for pediatric genetic disease testing and oncology. Read more to discover how she used NGS to speed up and expand sample processing while reducing costs.
More applications and methods
Detect transcriptional activity to resolve mRNA expression at the cellular level in structurally preserved tissues.
Analyze coding plus multiple forms of noncoding RNA for a comprehensive picture of the transcriptome.
Identify unique receptor variants to map the vast repertoire of immune cells capable of recognizing a seemingly boundless array of targets.
Sequence the entire genome of a bacterium, virus, or other microbe for applications in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.
Use sequence-specific hybridization to analyze genomic regions of interest.
Select and sequence specific transcripts of interest for gene expression profiling studies.