Targeted RNA-sequencing (RNA-Seq) is a highly accurate method for selecting and sequencing specific transcripts of interest. It offers both quantitative and qualitative information. Targeted RNA-Seq can be achieved via either enrichment or amplicon-based approaches, both of which enable gene expression analysis in a focused set of genes of interest. Enrichment assays also provide the ability to detect both known and novel gene fusion partners in many sample types, including formalin-fixed paraffin-embedded (FFPE) tissue.
RNA enrichment provides quantitative expression information as well as the detection of small variants and gene fusions. RNA enrichment offers the following features:
RNA expression panels can be designed to focus on RNA sequences of interest, or custom content can be added to fully optimized and experimentally validated panels.
Dr. Frank Middleton from SUNY Upstate Medical University (UMU) developed a focused gene panel to screen for expression alterations caused by a newly discovered CNV in schizophrenia. See why Dr. Middleton used targeted RNA-Seq rather than PCR or arrays to analyze several hundred genes associated with schizophrenia, bipolar disorder, and autism.Read Interview
Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of global sequencing data.*
We offer integrated targeted RNA-Seq workflows that simplify the entire process, from library preparation to data analysis and biological interpretation.
Click on the below to view products for each workflow step.
A highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds of genes in a single run.AmpliSeq for Illumina Custom RNA Panel
Targeted custom RNA research panels for sequencing up to 1200 targets of interest, starting with as little as 1-100 ng RNA. FFPE-compatible.TruSeq Targeted RNA Expression Kits
Highly customizable panels for mid- to high-plex gene expression profiling, starting with as little as 50 ng RNA. FFPE-compatible.TruSight RNA Pan-Cancer
Targeting 1385 oncology genes for gene expression, variant and fusion detection in many RNA sample types including FFPE.
Comprehensive panel detects single-nucleotide variants (SNVs), amplifications, and fusions that contribute to solid tumor progression.TruSight RNA Fusion
A gene fusion detection panel targeting fusion associated genes in many cancer types with the ability to detect known and novel fusion gene partners.DesignStudio Software
Easy-to-use, web-based assay design tool. Customize sequencing content to fit the needs of your study.Library Prep Kit Selector
Determine the best kit for your needs based on project type, starting material, and method or application.
Affordable, fast, and accessible sequencing power for targeted or small genome sequencing in any lab.MiniSeq System
Targeted power. Access proven Illumina quality with this small, robust NGS system.MiSeq Series
Focused power. Speed and simplicity for targeted and small genome sequencing.NextSeq 550 System
Flexible power. Speed and simplicity for everyday genomics.
Production power. High throughput and low cost for production-scale genomics.Platform Comparison Tool
Compare sequencing platforms and identify the best system for your lab and applications.Sequencing Reagents
Find kits that include sequencing reagents, flow cells, and buffers tailored to each Illumina sequencing system.
Analyzes RNA samples prepared using TruSeq Targeted RNA Expression Kits.BaseSpace RNA Amplicon App
Enables gene expression profiling using amplicon panels sequenced on Illumina platforms.Local Run Manager
Easy-to-use software for automated on-instrument data analysis.Integrative Genomics Viewer
A genome browser developed by the Broad Institute of MIT and Harvard that displays NGS data for complex variant analysis.
Associates single gene or list of genes with annotation data for pathways, diseases, tissues, and small molecules.iPathway Guide
Differential gene expression, drug interaction, and disease analysis.BaseSpace Sequence Hub
The Illumina genomics computing environment for NGS data analysis and management.BaseSpace Correlation Engine
A growing library of curated genomic data to support researchers in identifying disease mechanisms, drug targets, and biomarkers.
Monitor gene expression and transcriptome changes with targeted RNA-Seq to better understand which variants are expressed and which may affect tumorigenesis and progression. Learn more about RNA-Seq in cancer research.
Explore genomic sequencing solutions for all phases of the drug development pipeline. Characterize gene expression profiles from a custom panel with a few defined targets to the whole transcriptome. Learn more about NGS in drug development.
Gain insight into neurological disorders such as Alzheimer's and Parkinson's disease with specifically designed targeted RNA expression panels and arrays. Learn more about neuroscience research.
See how NGS enables research into autoimmune disease mechanisms, the immune repertoire, and the functional consequences of immune-related genetic variation. Learn more about immunology research.
*Data calculations on file. Illumina, Inc., 2015