Archival formalin-fixed paraffin-embedded (FFPE) samples hold an abundance of invaluable information for cancer studies. Because these samples generally yield highly degraded DNA, they typically perform poorly in array-based applications such as whole-genome genotyping. The Infinium HD FFPE DNA Restore Kit can repair these degraded DNA samples in preparation for use with the Infinium Assay.
Evaluate sample quality
After DNA extraction using one of many commercially available kits, the Illumina FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples. The assay utilizes standard instrumentation and reagents from an authorized vendor. Each kit provides primers and DNA template for 384 reactions.
Repair eligible FFPE samples
Extracted FFPE samples that pass the QC test are eligible for restoration using the Infinium HD FFPE Restore Kit. The protocol restores degraded FFPE DNA to an amplifiable state.
Product and species compatibility
Infinium FFPE QC and DNA Restoration Kits are designed to be used with Infinium BeadArray microarrays. The FFPE DNA Restore Kit is compatible with samples from any species, whereas the FFPE QC Kit is only compatible with human samples.