Ribo-Zero Gold rRNA Removal Kit (Epidemiology)

This kit removes cytoplasmic and mitochondrial rRNA from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA. Read More...
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Ribo-Zero Gold rRNA Removal Kit (Epidemiology) 6 reactions

MRZE706

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rRNA Removal Kit (Epidemiology) 24 reactions

MRZE724

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Product Highlights

  • Removes cytoplasmic and mitochondrial ribosomal RNA (rRNA) from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA (see table)
  • Requires 500 ng to 2.5 μg total RNA per sample
  • rRNA removal from both intact and partially degraded RNA
  • Single-pass, 90- to 120-minute process
  • Recovers all fragments of human and bacterial non-ribosomal RNAs
  • Applicable to a broad spectrum of bacteria

The Ribo-Zero Gold rRNA Removal Kit (Epidemiology) combines Ribo-Zero Gold (Human/Mouse/Rat) and Ribo-Zero (Bacteria) rRNA removal reagents into a single solution that effectively removes rRNA, including human mitochondrial RNA, from samples composed of both human/mouse/rat and bacterial RNA.

Hypothesis-free NGS is more sensitive to diversity than 16S profiling

Ribo-Zero Gold rRNA Removal Kit (Epidemiology) has increased sensitivity to biological diversity because it captures all metatranscriptomic information (instead of directed capture with 16S profiling).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads
Step 2: Treat samples with rRNA Removal Solution
Step 3: Remove rRNA
Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Related Information

rRNA Removal Kit Selection Guide
Ribo-Zero Kit Species Compatibility
Ribo-Zero Kit FAQs

Specifications

Ribo-Zero Gold (Epidemiology) Removes rRNA from Mixed Human/Bacteria Samples
% UHR E. coli RNA % B. subtilis RNA Human rRNAs % rRNA Removal E. coli rRNAs % rRNA Removal B. subtilis rRNAs % rRNA Removal
28S 18S 5.8S 5S 16S-mt 12S-mt 23S 16S 5S 23S 16S 5S
100 0 0 >99% >99% >99% >93% >99% >99%
0 100 0 >99% >99% >99%
0 0 100 >99% >99% >99%
90 5 5 >99% >99% >96% >92% >99% >99% >99% >99% >96% >99% >99% >95%
50 25 25 >99% >99% >99% >90% >99% >99% >99% >99% >97% >99% >99% >98%
10 45 45 >99% >99% >99% >88% >99% >99% >99% >99% >99% >99% >99% >99%

2.5 µg RNA samples, composed of the indicated proportion of human UHR, E.coli RNA and B. subtilis RNA, were treated using the Ribo-Zero Gold rRNA Removal Kit (Epidemiology). The percent rRNA removal was determined by random-primed qRT-PCR of the Ribo-Zero (Epidemiology)-treated RNA using PCR primers to the small rRNAs and to multiple regions of the large rRNAs. 16S-mt and 12S-mt are the 16S and 12S human mitochondrial rRNAs respectively.

Product Literature

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