|Organism||Gram +/-||% of Pool|
|Prochlorococcus marinus pastoris CMP 1986||-||1|
|Acinetobacter sp. ADP1||-||21|
|Cyanobacterium synechocystis PCC 6803||-||25|
|Synechococcus elongates PCC 7942||-||4|
A mixture of known bacteria containing gram-positive and gram-negative strains was created by Joint Genome Institute (JGI). The mixture was sequenced either without depletion (6.08 million reads) or after depletion by Ribo-Zero (Bacteria) (6.82 million reads). The percent of total reads that mapped to one of the bacterial strains increased 11.5-fold following depletion with Ribo-Zero (Bacteria).
Ribo-Zero (Bacteria) removes ribosomal RNA from gram-positive, gram-negative, and mixed samples.