This automated NGS-based proteomics solution with SOMAmer technology and Illumina sequencing and analysis detects and quantifies 9.5K human proteins in 2.5 days.
Illumina Protein Prep is available for Early Access
Early access to our innovative solution is now available for plasma and serum samples with a 9.5K unique human protein panel
Illumina Protein Prep enables discovery and quantification of human proteins across more than 200 biological pathways using a SOMAmer-based proteomics assay with an NGS readout.
SOMAmer Reagents used in the Illumina Protein Prep assay are rigorously characterized by shape specificity and polyionic competition and orthogonally confirmed by methods such as mass spectroscopy and ELISA (enzyme-linked immunosorbent assay).
SOMAmer Reagents provide market-leading reproducibility with ~5.5%* CV and femtomolar sensitivity with over a 10-log dynamic range.
*Expected median CV calculated using samples from healthy donors.
The entire workflow is automated on a single platform, providing an end-to-end solution for processing up to 340 samples and 44 controls per week, with seamlessly integrated secondary analysis pipelines and comprehensive tertiary analysis solutions.
| Assay time | ~2.5 days |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Automation details | The Illumina Protein Prep assay is fully automated on the Illumina Protein Prep Automation System, a custom Tecan Fluent 780, with the required parts to completely automate the assay on deck |
| Content specifications | The Illumina Protein Prep assay is the largest NGS-based proteomics panel with 9464 unique human protein targets |
| Description | Illumina Protein Prep is a NGS-based proteomics panel solution for the analysis of major molecular targets such as receptors, kinases, growth factors, and hormones spanning secreted, intracellular, and extracellular proteins in humans |
| Hands-on time | ~4 hr |
| Input quantity | 55 µL of human plasma or serum |
| Instruments | NovaSeq X System, NovaSeq 6000 System, NovaSeq X Plus System |
| Mechanism of action | Illumina Protein Prep uses the SomaScan assay for aptamer-based protein capture followed by Illumina library prep. The Illumina Protein Prep Automation System automates the entire assay workflow on deck |
| Method | NGS-based proteomics |
| Multiplexing | 96 reactions are run per batch on the Illumina Protein Prep Automation System; up to 384 reactions can be run on the NovaSeq X 25B flow cell, up to 192 reactions can be run on the NovaSeq X 10B flow cell and NovaSeq 6000 S4 flow cell |
| Sample type details | Compatible with human plasma or serum samples |
| Species category | Human |
| Technology | Sequencing |
Illumina Protein Prep Automation System (Catalog no. 20116818).
Required one-time purchase. The assay must be automated using the Illumina Protein Prep Automation System. Contact your local Illumina Account Manager to learn more about our Illumina Protein Prep service partners.
Choose either the plasma or serum assay based on your preferred input sample type:
Illumina Protein Prep 9.5K Plasma, 96 Reactions (Catalog no. 20137827), or Illumina Protein Prep 9.5K Serum, 96 Reactions (Catalog no. 20137828).
Choose one index plate per 96-reaction kit:
Illumina DNA/RNA UD Indexes (96 indexes, 96 samples)
Set A (Catalog no. 20141196)
Set B (Catalog no. 20141197)
Set C (Catalog no. 20141198)
Set D (Catalog no. 20141199)
Illumina Protein Prep enables sequence-ready libraries for the detection of 9464 unique human proteins for proteomic discovery and quantification applications, including biomarker discovery and population genetics studies.
Illumina Protein Prep
Illumina Protein Prep combines sequencing by synthesis chemistry with SOMAmer Reagents for a comprehensive NGS-based assay. The entire workflow is automated on the Illumina Protein Prep Automation System.
Gain unprecedented scalability and sequencing depth for comprehensive multiomic projects.
Access robust, scalable sequencing for high-throughput applications.
DRAGEN Protein Quantification
Integrated secondary analysis pipeline auto-launches after sequencing for complete normalization and protein expression quantification.
Illumina Connected Multiomics
Unlock deeper biological insights with a powerful omics analysis software featuring interactive visualization and statistical analysis.
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NovaSeq 6000 System | 192 reactions per flow cell on S4 35c |
40M reads, 1 × 15 |
| NovaSeq X System | 192 reactions per flow cell on 10B 100c |
40M reads, 1 × 15 |
Explore the entire set of proteins in an organism, also known as the proteome, with next-generation sequencing.
Use multiomics profiling to multiply discovery power and better connect genotype to phenotype.
A dedicated support section is not currently available for this product
Human serum or plasma samples are used as input to the SomaScan assay, followed by Illumina library prep, automated on Illumina Protein Prep Automation System, and sequenced on a NovaSeq System.
SOMAmer Reagents (blue) contain a photocleavable linker and biotin. Bound to streptavidin beads, they capture specific proteins and tag with biotin (yellow). UV light breaks the linker, releasing SOMAmer-protein complexes, which are captured on new beads and eluted for quantification using NGS.
Following the SomaScan assay, SOMAmer Reagent abundances are converted to barcoded sequencing-ready libraries using a hybridization-based approach.
SOMAmer technology (aptamer-based) is used for protein capture. See Protein capture using the SOMAScan assay in the Figures tab on this page.
Human plasma and serum samples.
The Illumina Protein Prep 9.5K assay detects 9464 unique human proteins. This panel can be used to detect proteins in major biological pathways, including those implicated in oncology, neurology, inflammation, and cardiology.
Secondary analysis generates a protein quantification file (ADAT) and DRAGEN QC report.
Read the data normalization technical note for more information on secondary analysis.
Yes, the assay must be automated using the Illumina Protein Prep Automation System.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.
Perform powerful high-throughput sequencing studies with the NovaSeq 6000 System. Match data output and time to results to project needs.
Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.
A rapid, cost-effective workflow for accurate, unbiased detection of the protein-coding transcriptome with precise measurement of strand information.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Reach out for information about our products and services, or get answers to questions about our technology.
Your email address is never shared with third parties.
