The HiSeq SR (Single Read) Cluster Kit v4 cBot provides reagents for the cBot cluster amplification system. Reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. DNA library samples are bound to complimentary adapter oligos grafted on the surface of the flow cell.
The templates are copied from the hybridization primer by 3’ extension using a high-fidelity DNA polymerase. Copies are isothermally amplified to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems*.