For use with HiSeq 2500, HiSeq 2000, HiSeq 1000, and HiScanSQ instruments with the cBot System, the TruSeq PE (Paired-End) Cluster Kit v3-cBot-HS provides reagents that bind samples to complementary adapter oligos on paired-end flow cells. These enable copying of the DNA strand after the first sequencing run, and the copied strand is used to sequence the other fragment end.
This kit enables cluster amplification on the cBot System. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.
Cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. The kit also includes reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.