For use with HiSeq 2500, HiSeq 2000, and HiSeq 1000 instruments with cBot, the TruSeq SR Cluster Kit v3-cBot-HS provides reagents that bind samples to complementary adapter oligos for cluster amplification on single-read flow cells.
The TruSeq SR Cluster Kit v3-cBot-HS contains cluster generation reagents for the cBot cluster amplification system in a pre-mixed, 96-well plate format that requires minimal reagent preparation.
DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.