The AmpliSeq for Illumina Exome Panel brings the speed and simplicity of PCR to exome sequencing, enabling researchers to sequence eight exomes in a single run and identify germline variants in less time. Key features include:
The Exome Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | Samples per run: high output: 8 samples per run (based on >95% covered at 30×) | 2 x 151 bp |
| AmpliSeq for Illumina Exome Panel | TruSeq DNA Exome | Nextera DNA Exome | Illumina DNA Prep with Enrichment | |
|---|---|---|---|---|
| Assay Time | < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 2.5 days | < 2.5 days | ~6.5 hours |
| Content Specifications | > 97% of CCDS | Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). | Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). | Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
| Description | Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. | A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. | A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. |
| Hands-On Time | < 1.5 hours | 6 hours | 3 hours | ~2 hours |
| Input Quantity | 50 ng minimum | 100 ng genomic DNA | 50 ng genomic DNA | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). |
| Mechanism of Action | Multiplex PCR | Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes | Transposase-based fragmentation and exome enrichment with biotinylated capture probes | Bead-bound transposomes and hybrid-capture chemistry |
| Method | Amplicon Sequencing , Exome Sequencing | Exome Sequencing | Exome Sequencing | Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations | Up to 12-plex enrichment | Up to 12-plex enrichment | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. |
| On-Target Reads | >80% | >80% | >75% | ≥85% (for exome panel)* |
| Specialized Sample Types | Not FFPE-Compatible | FFPE Tissue | Low-Input Samples, Not FFPE-Compatible | Blood, FFPE Tissue, Saliva |
| Species Category | Human | Human | Human | Human, Other |
| Uniformity | >90% (% coverage at 20x for 4 Gb) | >85% (% coverage at 20x for 4 Gb) | >75% (% coverage at 20x for 4 Gb) | ≥90% (% coverage at 20x for 4 Gb; for exome panel) |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
AmpliSeq for Illumina Exome Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Exome Panel Gene List
product_file | EXCEL< 1 MB
AmpliSeq for Illumina Exome Panel Reference Guide Documentation
AmpliSeq for Illumina Exome Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Exome Panel Checklist Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Exome Panel Documentation
Illumina Adapter Sequences Document Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
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Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
