AmpliSeq for Illumina Exome Panel

Targeted research panel for sequencing the protein-coding regions of the human genome.Read More...
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Panel

AmpliSeq™ Exome Panel for Illumina®

20019166

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

Ampliseq™ CD Indexes Large Volume for Illumina® (96 Indexes, 96 Samples)

20019108

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Accessory Products

AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Exome Panel brings the speed and simplicity of PCR to exome sequencing, enabling researchers to sequence eight exomes in a single run and identify germline variants in less time. Key features include:

Fast exome library preparation
  • Prepare 8 exome libraries in < 7.5 hours with < 1.5 hours hands-on time
Comprehensive coverage
  • Access uniform and specific coverage; includes >97% of the Consensus Coding Sequences (CCDS) annotation
Accurate Data
  • Detect common single nucleotide variants (SNVs), copy number variants (CNVs), and small indels

The Exome Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System Samples per run: high output: 8 samples per run (based on >95% covered at 30×) 2 x 151 bp

Product Comparison

AmpliSeq for Illumina Exome Panel TruSeq DNA Exome Nextera DNA Exome Nextera Flex for Enrichment
Assay Time < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 2.5 days < 2.5 days ~6.5 hours
Content Specifications > 97% of CCDS Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Description Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.
Hands-On Time < 1.5 hours 6 hours 3 hours ~2 hours
Input Quantity 50 ng minimum 100 ng genomic DNA 50 ng genomic DNA 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Multiplex PCR Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes Transposase-based fragmentation and exome enrichment with biotinylated capture probes Enrichment-compatible, bead-linked transposome
Method Amplicon Sequencing , Exome Sequencing Exome Sequencing Exome Sequencing Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing
Multiplexing 96 dual index combinations Up to 12-plex enrichment Up to 12-plex enrichment Up to 96 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.
On-Target Reads >80% >80% >75% ≥85% (for exome panel)
Specialized Sample Types Not FFPE-Compatible FFPE Tissue Low-Input Samples, Not FFPE-Compatible Blood, FFPE Tissue, Saliva
Species Category Human Human Human Human, Other
Uniformity >90% (% coverage at 20x for 4 Gb) >85% (% coverage at 20x for 4 Gb) >75% (% coverage at 20x for 4 Gb) ≥90% (% coverage at 20x for 4 Gb; for exome panel)

Method-Specific Workflow Example

 

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