Targeted gene sequencing panels are useful tools for analyzing specific mutations in a given sample. Focused panels contain a select set of genes or gene regions that have known or suspected associations with the disease or phenotype under study. Gene panels can be purchased with preselected content or custom designed to include genomic regions of interest.
Next-generation sequencing (NGS) offers the scalability, speed, and resolution to evaluate targeted genes of interest. Multiple genes can be assessed across many samples in parallel, saving time and reducing costs associated with running multiple separate assays. Targeted gene sequencing also produces a smaller, more manageable data set compared to broader approaches such as whole-genome sequencing, making analysis easier.
Predesigned panels contain important genes or gene regions associated with a disease or phenotype, selected from publications and expert guidance. By focusing on the genes most likely to be involved, these panels conserve resources and minimize data analysis considerations. Predesigned panels are available for research on various diseases, such as cancer, inherited disorders, cardiac conditions, and autism.View Panels
With custom designs, researchers can target regions of the genome relevant to their specific research interests. Custom targeted sequencing is ideal for examining genes in specific pathways, or for follow-up experiments from genome-wide association studies or whole-genome sequencing.
Illumina supports two methods for targeted sequencing: target enrichment and amplicon generation.
DesignStudio: An easy-to-use online software tool that provides dynamic feedback to optimize probe designs.
TruSeq Custom Amplicon Library Prep Kit: Fully customizable, amplicon-based assay for targeted resequencing.
Nextera Rapid Capture Custom Enrichment Kit: Custom assay for enriching genomic regions of interest, with add-on functionality.
Amplicon Sequencing: Learn more about this method, and find seamless workflow solutions.
|Larger gene content, typically > 50 genes||Smaller gene content, typically < 50 genes|
|More comprehensive profiling for all variant types||Ideal for analyzing single nucleotide variants and insertions/deletions (indels)|
|More comprehensive method, but with longer hands-on time and turnaround time*||More affordable, easier workflow|
* the turnaround time is for library prep assay time (DNA to finished library).
Next-generation sequencing (NGS) offers the scalability, speed, and resolution for targeted resequencing. Our Gene Panel Finder will help you identify predesigned Illumina sequencing panels to match your genes of interest.Find the Right Panel
Targeted cancer panels focus on a select set of genes or gene regions that have known associations with cancer. The deep coverage offered by targeted resequencing studies enables high sensitivity for rare variant calling in all sample types, including formalin-fixed, paraffin-embedded (FFPE) tissue. Learn more about targeted cancer sequencing.
Sequencing genes that have known involvement in certain disorders or conditions, such as autism or cardiovascular disease, can detect variants that contribute to inherited disorders. NGS can reduce costs and shorten the time to answer compared to traditional, iterative approaches. Learn more about inherited condition screening with NGS.
Achieving high-resolution human leukocyte antigen (HLA) typing results with conventional methods requires multiple assays, multiple systems, and multiple analysis programs. In contrast, HLA typing research with NGS generates phase-resolved results using a single assay. Learn more about HLA sequencing.