Illumina Custom Enrichment Panel v2

Targeted custom enrichment panels, providing simple and efficient solutions for multiple targeted sequencing applications.

Illumina Custom Enrichment Panel v2

Targeted custom enrichment panels, providing simple and efficient solutions for multiple targeted sequencing applications.

Illumina DNA Prep with Exome 2.0 Enrichment data sheet
Data sheet PDF < 1 MB

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Overview
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Overview

Illumina Custom Enrichment Panel v2 provide fast, flexible content for targeted sequencing applications, enabling a fully customized enrichment solution.

Key features

Design your custom panel easily with DesignStudio, a free online tool
Create panels specific for your content of interest, with dynamic feedback to optimize target coverage
Design complete custom panels, spike-in panels for addition to exome or other panels, or modify one of our fixed panel designs to meet your needs
Enable non-human species designs with support from Illumina Concierge design team
Single, simple enrichment solution

Flexible, scalable solution

Design large or small panels, from 100 to 1,000,000 probes
Order the volume that’s right for your experiments–from as little as 32 µl of probe panel to larger volumes for automated library prep workflows

Learn more about Targeted Enrichment

Specifications

Description	Fully customized enrichment panels with speed, flexibility, and scalability for targeted sequencing applications.
Instruments	NovaSeq 6000Dx Instrument, MiSeq System, MiSeqDx Instrument, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System
Library prep compatibility	Illlumina Cell-Free DNA Prep with Enrichment, Illumina Complete Long Reads with Enrichment, Human, Illlumina DNA Prep with Enrichment
Method	Target enrichment
Nucleic acid type	DNA
Species category	Any species, Other, Human
Technology	Sequencing

Required Products

Illumina Custom Enrichment Panel v2 is a 120 bp, double-stranded probe panel designed using DesignStudio and includes enrichment oligos for hybridization capture. Library prep, enrichment and index adapter reagents must be purchased separately.

/ Results

Applications

Illumina Custom Enrichment Panel v2 is compatible with multiple targeted enrichment workflows. With DesignStudio, panels can be designed for use with multiple Illumina library preps:

Example workflow

Library Preparation

Sequencing

Data Analysis

Related applications and methods

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Illumina DNA Prep technology provides time-saving solutions for optimized library preparation across a wide range of sequencing applications.

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Documentation

Product literature

Illumina Complete Long Read Prep with Enrichment, Human data sheet
Data sheet PDF 2 MB

Illumina DNA Prep with Enrichment
Data sheet PDF < 1 MB

Illumina DNA Prep with Exome 2.0 Enrichment data sheet
Data sheet PDF < 1 MB

Support documentation

A dedicated support section is not currently available for this product

Figures

Illumina Custom Enrichment Panel on-target rates

Illumina Custom Enrichment Panel v2 with Illumina DNA Prep with Enrichment provides higher padded read enrichment and mean target coverage.

Illumina Custom Enrichment Panels v2 - flexible, scalable custom enrichment panels with simple DIY design

Estimated number of samples expected per flow cell based on probe panel size

Custom panel sample calculations assume the indicated target coverage depths using 120 bp double-stranded DNA oligo panels (Illumina Custom Enrichment Panel v2) or 80-mer single-stranded dNA oligo panels (Illumina Custom Enrichment Panel) for target capture at 80% enrichment efficiency and 2 x 101 bp read length chemistry. Number of samples may vary depending on workflow handling, input sample or library quality, and actual sequencing output of each platform and flow cell.

Compare

SKU comparison

Part Number	Name	Volume (µl)	# of Samples: Illumina DNA Prep with Enrichment	# of Samples: Illumina Cell-Free DNA Prep with Enrichment	# of Samples: Illumina Complete Long Reads Prep with Enrichment
20073953	Illumina Custom Enrichment Panel v2 (32 µl, 120 bp)	32 µl	96	8 (1-plex) or 32 (4-plex)	64
20073952	Illumina Custom Enrichment Panel v2 (384 µl, 120 bp)	384 µl	1152	96 (1-plex) or 384 (4-plex)	768
20111339	Illumina Custom Enrichment Panel v2 (1536 µl, 120 bp)	1536 µl	4608	384 (1-plex) or 1536 (4-plex)	3072
		Panel Volume per Hyb	4 ul probe panel per hyb rxn	4 ul probe panel per hyb rxn	Dilute probe panel 5-fold, use 2.5 ul per hyb
		Plexity	12 samples per hyb*	1 sample or 4 samples per hyb	1 sample per hyb

	Illumina Custom Enrichment Panel v2	Illumina Custom Enrichment Panel
Panel size, # of probes	100 to 1,000,000	2000 to 67,000
Oligo format	120 bp double-stranded oligo probes	80-mer single-stranded oligo probes
Available dispense sizes	32 µl, 384 µl, 1536 µl	80 µl
>99.9% of probes present, confirmed by NGS	Yes	No
Compatible Illumina Library Preps	Illumina DNA Prep with Enrichment, Illumina Cell-Free DNA Prep with Enrichment, Illumina Exome 2.5 Enrichment, Illumina Complete Long Reads Enrichment	Illumina DNA Prep with Enrichment, Illumina Cell-Free DNA Prep with Enrichment
Design Tool	DesignStudio or Concierge Services	DesignStudio or Concierge Services
Turn-around time*	3-6 weeks	6-8 weeks
Panel Cost	$	$$$

^*additional ship time may be needed for non-US destinations

Selection tools:

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FAQs

Illlumina has supported protocols for use of Illumina Custom Enrichment v2 panels with Illumina DNA Prep with Enrichment and Illumina DNA Prep with Exome 2.5 Enrichment, Illumina cfDNA Prep with Enrichment and Illumina Complete Long Reads with Enrichment workflows.

	Illumina DNA Prep with Enrichment	Illumina DNA Prep with Exome 2.5 Enrichment	Illumina cfDNA Prep with Enrichment	Illumina Complete Long Reads with Enrichment
Minimum panel size	500 probes	100 probes (when combined with Exome 2.5)	70kb or 700 probes, for double-stranded probes	2,500 (0.3Mb panel with 190 probes was tested however PRE=34.8%)
Supported plexities	1-plex or 12-plex	12-plex	1-plex or 4-plex	1-plex
Volume of Panel used	4 µl per enrichment reaction	4 µl per enrichment reaction	4 µl per enrichment reaction	Dilute panel 5-fold, use 2.5 µl per enrichment reaction
# of samples supported (20073952 Illumina Custom Enrichment Panel v2 (32 µl, 120-bp)	8 samples at single-plex 96 samples at 12-plex	96 samples at 12-plex	8 samples at single-plex 96 samples at 12-plex	64 samples at 1-plex
# of samples supported (20073953 Illumina Custom Enrichment Panel v2 (384 µl, 120-bp)	96 samples at single-plex 1,152 samples at 12-plex	1,152 samples at 12-plex	96 samples at 1-plex 384 samples at 4-plex	768 samples at 1-plex

Illlumina has supported protocols for use of Illumina Custom Enrichment panels with Illumina DNA Prep with Enrichment and Illumina cfDNA Prep with Enrichment workflows.

The sample type compatibility is determined by the library prep kit paired with the custom panel. For Early Access we have supported protocols for use of the panels with Illumina DNA Prep with Enrichment (which is compatible with saliva, blood, purified gDNA and FFPE) and Illumina DNA Prep with Exome 2.5 Enrichment (saliva, blood and purified gDNA). Library prep and enrichment protocols with defined supported sample types will be released with launch of the Illumina cfDNA Prep with Enrichment and Illumina Complete Long Read with Enrichment workflows.

The Illumina Concierge Design Team can help with complex designs, or designs for enrichment panels for non-human targets. This is a free service open to all customers. Contact your Illumina Sales Team representative to get connected to the Concierge team.

/ Results

Panel (3)

Illumina Custom Enrichment Panel V2 (32 µl, 120 bp)

20073953

Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 32 µl of material (sufficient for 8 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately.

Customize

List Price:

Discounts:

Illumina Custom Enrichment Panel V2 (384 µl, 120 bp)

20073952

Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 384 µl of material (sufficient for 96 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately.

Customize

List Price:

Discounts:

Illumina Custom Enrichment Panel V2 (1536 µl, 120 bp)

20111339

Illumina Custom Enrichment Panel V2 enables 120 bp double-stranded custom panel design from 100 up to 1,000,000 probes using DesignStudio or Concierge Services and provides 1536 µl of material (sufficient for 384 enrichment reactions when paired with Illumina DNA Prep with Enrichment). Library prep, enrichment, and index adapter reagents need to be ordered separately.

Customize

List Price:

Discounts:

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Illumina Custom Enrichment Panel v2

Illumina Custom Enrichment Panel v2

Overview

Key features

Flexible, scalable solution

Specifications

Required Products

What products do I need?

Applications

Example workflow

Library Preparation

Sequencing

Data Analysis

Related applications and methods

Documentation

Product literature

Support documentation