| Assay Time |
6.5 hours |
~6.5 hours |
2.5 days |
| Content Specifications |
Twist Bioscience for Illumina Exome 2.5 Panel
37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM
Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes) |
Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel. |
Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). |
| Description |
A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing. |
A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. |
A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. |
| Hands-On Time |
~2 hours |
~2 hours |
6 hours |
| Input Quantity |
50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) |
10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). |
100 ng genomic DNA |
| Mechanism of Action |
Bead-linked transposome and hybrid-capture chemistry |
Bead-bound transposomes and hybrid-capture chemistry |
Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes |
| Method |
Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
Exome Sequencing |
| Multiplexing |
Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. |
Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. |
Up to 12-plex enrichment |
| Nucleic Acid Type |
DNA |
DNA |
DNA |
| On-Target Reads |
~ 90% (ave.) based on average performance from internal testing |
≥85% (for exome panel)* |
>80% |
| Specialized Sample Types |
Blood, Saliva |
Blood, FFPE Tissue, Saliva |
FFPE Tissue |
| Species Category |
Human |
Human, Other |
Human |
| System Compatibility |
HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus |
HiSeq 3000, HiSeq 4000, iSeq 100, MiniSeq, MiSeq, MiSeqDx in Research Mode, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus |
HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000, NovaSeq X, NovaSeq X Plus |
| Technology |
Sequencing |
Sequencing |
Sequencing |
| Uniformity |
≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing |
≥90% (% coverage at 20x for 4 Gb; for exome panel) |
>85% (% coverage at 20x for 4 Gb) |
| Variant Class |
Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants |
Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants |
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