Illumina Purification Beads, 100ml
Illumina Purification Beads, 400ml
|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 2000 System||Samples per run (by flow cell type): P2: 19, P3: 57 (based on 50× mean target coverage, 42M reads, 4.2 Gb of data)||2 × 101 bp|
|NovaSeq 6000 System||Samples per run by flow cell type: SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes.||2 × 101 bp|
|Illumina DNA Prep with Exome 2.5 Enrichment||Illumina DNA Prep with Enrichment||TruSeq DNA Exome|
|Assay Time||6.5 hours||~6.5 hours||2.5 days|
|Content Specifications||Twist Bioscience for Illumina Exome 2.5 Panel
37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM
Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes)
|Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).|
|Description||A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.||A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.|
|Hands-On Time||~2 hours||~2 hours||6 hours|
|Input Quantity||50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide)||10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).||100 ng genomic DNA|
|Mechanism of Action||Bead-linked transposome and hybrid-capture chemistry||Bead-bound transposomes and hybrid-capture chemistry||Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes|
|Method||Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing||Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing||Exome Sequencing|
|Multiplexing||Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible.||Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.||Up to 12-plex enrichment|
|Nucleic Acid Type||DNA||DNA||DNA|
|On-Target Reads||~ 90% (ave.) based on average performance from internal testing||≥85% (for exome panel)*||>80%|
|Specialized Sample Types||Blood, Saliva||Blood, FFPE Tissue, Saliva||FFPE Tissue|
|Species Category||Human||Human, Other||Human|
|System Compatibility||HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus||HiSeq 3000, HiSeq 4000, iSeq 100, MiniSeq, MiSeq, MiSeqDx in Research Mode, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus||HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000, NovaSeq X, NovaSeq X Plus|
|Uniformity||≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing||≥90% (% coverage at 20x for 4 Gb; for exome panel)||>85% (% coverage at 20x for 4 Gb)|
|Variant Class||Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants||Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants|
* Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
High performance of Illumina DNA Prep with Exome 2.5 Enrichment which is improved compared to the original Illumina Exome Panel. Libraries were constructed and enriched using Illumina DNA Prep with Exome 2.5 Enrichment kit with either the included Exome 2.5 Panel or the Illumina Exome Panel (IEP, 20020183) with hybridization (1.5 or 16 hr, 1.5 hr for Illumina Exome Panel) and washing temperatures of 62°C for Exome 2.0 Plus or 58°C for Illumina Exome Panel. Enriched libraries were sequenced on a NovaSeq 6000/S4 flow cell using 2 x 101 bp chemistry, and 50M reads per enriched library were analyzed using DRAGEN and the average results presented.
High database target coverage rates both in silico and experimentally. The bed file for the Exome 2.0 Plus Panel was compared to the listed public databases and the percent overlap was determined and reported. For the experimental data, libraries were constructed and enriched using the Illumina DNA Prep with Exome 2.0 Plus with Exome 2.0 Plus kit using a 1.5 hr (orange) or 16-hour (blue) hybridization time and washing temperatures of 62°C. Enriched libraries were sequenced on a NovaSeq 6000/S4 flow cell with 2 x 101 bp chemistry, and 50M reads per enriched library were analyzed using DRAGEN and the average results presented.
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