Illumina Purification Beads, 100ml
20060057
Illumina Purification Beads, 400ml
20060058
Illumina DNA Prep with Exome 2.5 Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES).
The Twist Bioscience for Illumina Mitochondrial Panel is a fixed content panel designed to cover the 16,659 base pairs (bp) and 37 genes of the mitochondrial genome (chrM) and allows enrichment and sequencing of mitochondrial DNA (mtDNA) variants. Use this panel as a spike-in with the Twist Bioscience for Illumina Exome 2.5 Panel and add coverage of ChrM to your experiments, with the same high WES on-target rates and coverage uniformity.
Illumina DNA Prep with Exome 2.5 Enrichment provides high on-target rates and uniform coverage enabling high multiplexing, in turn decreasing costs and increasing sample throughput.
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
Illumina DNA Prep with Exome 2.5 Enrichment uses a fast, user-friendly workflow with built-in library yield normalization and a simplified, single hybridization protocol. Go from DNA to library prep, enrichment, and post-enrichment amplification in just 6.5 hours.
You and your lab can benefit from a WES solution with support from a single, reliable partner—starting from direct blood or saliva input, through library prep, enrichment, sequencing, analysis, interpretation, and reporting.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
NextSeq 2000 System | Samples per run (by flow cell type): P2: 19, P3: 57 (based on 50× mean target coverage, 42M reads, 4.2 Gb of data) | 2 × 101 bp |
NovaSeq 6000 System | Samples per run by flow cell type: SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes. | 2 × 101 bp |
Illumina DNA Prep with Exome 2.5 Enrichment | Illumina DNA Prep with Enrichment | TruSeq DNA Exome | |
---|---|---|---|
Assay Time | 6.5 hours | ~6.5 hours | 2.5 days |
Content Specifications | Twist Bioscience for Illumina Exome 2.5 Panel 37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes) |
Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). |
Description | A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. | A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. |
Hands-On Time | ~2 hours | ~2 hours | 6 hours |
Input Quantity | 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). | 100 ng genomic DNA |
Mechanism of Action | Bead-linked transposome and hybrid-capture chemistry | Bead-bound transposomes and hybrid-capture chemistry | Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes |
Method | Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Exome Sequencing |
Multiplexing | Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. | Up to 12-plex enrichment |
Nucleic Acid Type | DNA | DNA | DNA |
On-Target Reads | ~ 90% (ave.) based on average performance from internal testing | ≥85% (for exome panel)* | >80% |
Specialized Sample Types | Blood, Saliva | Blood, FFPE Tissue, Saliva | FFPE Tissue |
Species Category | Human | Human, Other | Human |
System Compatibility | HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus | HiSeq 3000, HiSeq 4000, iSeq 100, MiniSeq, MiSeq, MiSeqDx in Research Mode, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000, NovaSeq X, NovaSeq X Plus | HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000, NovaSeq X, NovaSeq X Plus |
Technology | Sequencing | Sequencing | Sequencing |
Uniformity | ≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing | ≥90% (% coverage at 20x for 4 Gb; for exome panel) | >85% (% coverage at 20x for 4 Gb) |
Variant Class | Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants | Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants |
Data Sheet | PDF | 6 versions
Custom Protocol Selector
Generates customized, end-to-end instructions
We'll walk through your needs and make recommendations.