The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:
- Assemble synthetically long reads for de novo assembly and genome finishing applications
- Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations
Accurately Construct Synthetic Long Reads
The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.
Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing
After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.
BaseSpace App for Genome Assembly:
The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.
BaseSpace App for Phasing Analysis:
The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.