Advantages of RNA-Seq technology

Wider dynamic range and higher sensitivity than microarrays, with novel transcript detection

RNA-Seq and Microarray Technology Comparison

RNA sequencing (RNA-Seq) technology enables rapid profiling and deep investigation of the transcriptome, for any species. This approach offers a number of advantages compared to microarray analysis, a legacy technology often used in gene expression studies.

RNA-Seq Technology Overview

Unbiased detection of novel transcripts: Unlike arrays, RNA-Seq technology does not require species- or transcript-specific probes. It can detect novel transcripts, gene fusions, single nucleotide variants, indels (small insertions and deletions), and other previously unknown changes that arrays cannot detect.

Broader dynamic range: With array hybridization technology, gene expression measurement is limited by background at the low end and signal saturation at the high end. RNA-Seq technology quantifies discrete, digital sequencing read counts, offering a broader dynamic range.

Increased specificity and sensitivity: Compared to microarrays, RNA-Seq technology offers increased specificity and sensitivity, for enhanced detection of genes, transcripts, and differential expression.

Easier detection of rare and low-abundance transcripts: Sequencing coverage depth can easily be increased to detect rare transcripts, single transcripts per cell, or weakly expressed genes.

Transitioning from Arrays to RNA-Seq

Access a detailed comparison of microarray and RNA-Seq technologies, from the perspective of a sequencing service provider.

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In the past, next-generation sequencing (NGS) data analysis required extensive bioinformatics expertise, presenting a major hurdle to adoption of RNA sequencing technology by biologists. The latest user-friendly tools vastly simplify the analysis process, providing accessible solutions for any researcher.

Explore RNA-Seq Data Analysis Tools

Benchtop RNA-Seq Technology

The NextSeq Series provides a scalable approach for RNA-Seq, with higher sensitivity and accuracy than conventional arrays.

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The portion of grant funding allocated to new RNA sequencing vs. gene expression microarray-inclusive grants has been trending towards RNA-Seq technology for the last several years, and now constitutes the majority. See the evidence in the blog article below.

Read Blog Post: Trends in Transcriptomics
Empowering Transcriptomics

This ebook discusses how NGS is advancing gene expression research, and includes a section on the advantages of RNA-Seq over arrays.

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RNA Sequencing Considerations

Each RNA-Seq experiment type—whether it’s gene expression profiling, targeted RNA expression, or small RNA analysis—has unique requirements for read length and depth. This bulletin reviews experimental considerations and offers resources to help with study design.

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RNA Sequencing Considerations

Explore a wide variety of RNA-Seq methods, from mRNA-Seq to specialized methods for analyzing RNA from single cells and more.

See All RNA-Seq Methods

Gene Expression Profiling and Validation

TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation.

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