Advantages of RNA-Seq technology

Wider dynamic range and higher sensitivity than microarrays, with novel transcript detection

RNA-Seq and Microarray Technology Comparison

RNA sequencing (RNA-Seq) technology enables rapid profiling and deep investigation of the transcriptome, for any species. This approach offers a number of advantages compared to microarray analysis, a legacy technology often used in gene expression studies.

RNA-Seq Technology Overview

Unbiased detection of novel transcripts: Unlike arrays, RNA-Seq technology does not require species- or transcript-specific probes. It can detect novel transcripts, gene fusions, single nucleotide variants, indels (small insertions and deletions), and other previously unknown changes that arrays cannot detect.

Broader dynamic range: With array hybridization technology, gene expression measurement is limited by background at the low end and signal saturation at the high end. RNA-Seq technology quantifies discrete, digital sequencing read counts, offering a broader dynamic range.

Increased specificity and sensitivity: Compared to microarrays, RNA-Seq technology offers increased specificity and sensitivity, for enhanced detection of genes, transcripts, and differential expression.

Easier detection of rare and low-abundance transcripts: Sequencing coverage depth can easily be increased to detect rare transcripts, single transcripts per cell, or weakly expressed genes.

Transitioning from Arrays to RNA-Seq

Access a detailed comparison of microarray and RNA-Seq technologies, from the perspective of a sequencing service provider.

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In the past, next-generation sequencing (NGS) data analysis required extensive bioinformatics expertise, presenting a major hurdle to adoption of RNA sequencing technology by biologists. The latest user-friendly tools vastly simplify the analysis process, providing accessible solutions for any researcher.

Explore RNA-Seq Data Analysis Tools

Benchtop RNA-Seq Technology

The NextSeq Series provides a scalable approach for RNA-Seq, with higher sensitivity and accuracy than conventional arrays.

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The portion of grant funding allocated to new RNA sequencing vs. gene expression microarray-inclusive grants has been trending towards RNA-Seq technology for the last several years, and now constitutes the majority. See the evidence in the blog article below.

Read Blog Post: Trends in Transcriptomics
Empowering Transcriptomics

This ebook discusses how NGS is advancing gene expression research, and includes a section on the advantages of RNA-Seq over arrays.

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Explore a wide variety of RNA-Seq methods, from mRNA-Seq to specialized methods for analyzing RNA from single cells and more.

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Gene Expression Profiling and Validation

TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation.

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