Nextera DNA Indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput.
Nextera DNA Indexes enable multiplexing of high-quality sequencing libraries generated from the Nextera XT DNA Library Preparation Kit or Illumina DNA Prep. Available in two adapter kits, the Nextera XT Index Kit v2 and Nextera DNA CD Indexes suit a range of throughput next-generation sequencing (NGS) applications.
Nextera DNA Indexes are combinatorial dual (CD) indexes and cannot eliminate index hopping artifacts during demultiplexing. CD indexes work best for applications that do not require high sensitivity and for multiplexing on sequencing systems that use nonpatterned flow cells, such as the MiniSeq, MiSeq, and NextSeq 550 Systems.
It is recommended to use Illumina DNA/RNA UD Indexes for multiplexing on sequencing platforms with patterned flow cells such as the NextSeq 2000 System, NovaSeq 6000 System, and NovaSeq X Series to reduce index hopping or read misassignment during demultiplexing. Nextera XT v2 indexes and Nextera Combinatorial Dual (CD) indexes cannot eliminate read misassignment due to index hopping artifacts.
Find kits compatible with Nextera DNA indexes and learn more about how to minimize index hopping on your next sequencing run.
| Instruments | MiSeq System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System |
|---|---|
| Mechanism of action |
PCR-based adapters |
| Multiplexing | Up to 384-plex |
The Nextera XT Index Kit v2 Sets A–D and Nextera XT Index Kit are compatible with the Nextera XT DNA Library Preparation Kit. The library preparation kit needs to be ordered separately.
The Nextera DNA CD indexes are compatible with Illumina DNA Prep. The library preparation kit needs to be ordered separately.
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Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)
FC-131-2001
Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.
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Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)
FC-131-2002
Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.
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Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)
FC-131-2003
Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.
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Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)
FC-131-2004
Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.
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Nextera XT Index Kit (24 indexes, 96 samples)
FC-131-1001
Includes 24, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and purification beads separately.
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Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)
20018708
Includes 20 indexes, eight of i500s and 12 of i700 indexes that together provide 96 dual indexing capability sufficient for labeling 96 samples. Indexes are provided in a 96 well format. Purchase library prep and probe panels separately.
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The Illumina Adapter Sequences Document lists index adapter sequences and all other adapter sequences.
Visiit our multiplex sequencing page for more information.
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A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Unique dual (UD) indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput and cost-saving benefits.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
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