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TruSight Cancer

These expert-defined sequencing research panels target 94 genes and 284 SNPs associated with a predisposition towards various cancers.Read More...
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TruSight Cancer – Enrichment Oligos only (4 or 8 Enrichment Reactions)

FC-121-0202

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TruSight Cancer MiniSeq Kit

20005612

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Accessory Products
What accessories do I need?

Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)

20025524

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Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 16 samples (16, 1-plex enrichment reactions)

20025523

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (96 samples)

20025520

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (16 samples)

20025519

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IDT® for Illumina Nextera DNA UD Indexes Set A (96 Indexes, 96 Samples)

20027213

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TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)

FC-140-1101

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TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)

FC-140-1102

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TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)

FC-140-1103

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TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)

FC-140-1104

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TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)

FC-140-1105

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TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)

FC-140-1106

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Product Highlights

Developed in collaboration with cancer genomics experts, these predesigned, ready-to-use oligos enable researchers to sequence a variety of genes and single nucleotide polymorphisms (SNPs) previously linked to cancer predisposition.

  • Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatible with TruSight Rapid Capture.

TruSight Cancer Sample Datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

* Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

See All Illumina Cancer Research Panels

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run: mid output: 12, high output: 24 (based on > 20x coverage per target) Up to 2 × 150 bp
MiSeq System Samples per run (by reagent kit version): v2: 12, v3: 24 (based on > 20x coverage per target) Up to 2 × 150 bp
NextSeq 550 System Samples per run: mid output: 96, high output: 96 (based on > 20x coverage per target) Up to 2 × 150 bp

Product Comparison

TruSight Cancer AmpliSeq for Illumina BRCA Panel
Assay Time 1.5 days 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Pan-Cancer Solid Tumor
Content Specifications Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2
Description Germline mutation detection research for common and rare cancers. Germline and somatic analysis studies of BRCA1 and BRCA2 .
Hands-On Time 5 hours < 1.5 hours
Input Quantity 50 ng DNA 1–100 ng (10 ng recommended per pool)
Method Target Enrichment , Target Enrichment, Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing Up to 96-plex 96 dual index combinations
Specialized Sample Types Not FFPE-Compatible FFPE Tissue
Species Category Human Human
Variant Class Germline Variants Germline Variants, Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Somatic Variants

Method-Specific Workflow Example

 

Customer Stories

From Arrays to NGS: How Chromosomal Genetics Evolved From Structure to Disease

Researchers from the MGZ Medical Genetic Center are using the Infinium CytoSNP-850K BeadChip and the TruSight Cancer Panel to analyze chromosomal abnormalities.

Read More

Related Products

Nextera Flex for Enrichment

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AmpliSeq for Illumina Custom DNA Panel

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.


AmpliSeq for Illumina BRCA Panel

Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.


Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.