Reagent kits that provide increased cluster density and read length for the MiSeq System, improving sequencing quality scores compared to v1 kits.
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits but offer improvements in data quality and allow various formats for low-output applications.
MiSeq Reagent Kit |
Single readsa |
Paired-end readsa |
Kit size (cycles) |
Output (max.) |
2 × 250 Output |
2 × 150 Output |
2 × 25 Output |
1 × 36 Output |
| MiSeq Reagent Kit v2 | 12–15M | 24–30M |
50, 300, 500 |
8.5 Gb |
7.5–8.5 Gb |
4.5–5.1 Gb |
750–850 Mb |
540–610 Mb |
MinSeq Reagent Micro Kit v2 |
4M |
8M | 300 |
1.2 Gb |
N/A |
1.2 Gb |
N/A |
N/A |
MiSeq Reagent Nano Kit v2 |
1M |
2M |
300, 500 |
500 Mb |
500 Mb |
300 Mb |
N/A |
N/A |
a. Install specifications based on Illumina PhiX control library at supported cluster densities between 467–583 k/mm2 clusters passing filter for v2 chemistry and 727–827 k/mm2 clusters passing filter for v3 chemistry. Actual performance parameters may vary based on library type, library quality, and clusters passing filter.
| Instruments | MiSeq System, MiSeqDx in Research Mode |
|---|---|
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis |
| Technology | Sequencing |
AmpliSeq for Illumina Cancer Hotspot Panel v2
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
AmpliSeq for Illumina Focus Panel
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
AmpliSeq for Illumina Immune Response Panel
Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.
AmpliSeq for Illumina BRCA Panel
Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
View a demonstration of the steps to prepare the MiSeq Reagent Cartridge prior to sequencing.
View a demonstration of the steps to handle and clean the MiSeq flow cell prior to sequencing.
MiSeq Reagent Kit v2 (50-cycles)
MS-102-2001
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 50-cycle run on the MiSeq System.
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MiSeq Reagent Kit v2 (300-cycles)
MS-102-2002
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the MiSeq System.
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MiSeq Reagent Kit v2 (500-cycles)
MS-102-2003
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 500-cycle run on the MiSeq System.
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20-pack MiSeq Reagent Kit v2 (50-cycles)
MS-102-2021
Includes 20 kits each with one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support (20) 50-cycle runs on the MiSeq System.
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20-pack MiSeq Reagent Kit v2 (300-cycles)
MS-102-2022
Includes 20 kits each with one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support (20) 300-cycle runs on the MiSeq System.
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20-pack MiSeq Reagent Kit v2 (500-cycles)
MS-102-2023
Includes 20 kits each with one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support (20) 500-cycle runs on the MiSeq System.
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MiSeq Reagent Micro Kit v2 (300-cycles)
MS-103-1002
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.
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MiSeq Reagent Nano Kit v2 (300-cycles)
MS-103-1001
Includes one Nano flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the MiSeq System.
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MiSeq Reagent Nano Kit v2 (500-cycles)
MS-103-1003
Includes one Nano flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 500-cycle run on the MiSeq System.
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TG MiSeq Reagent Nano Kit, v2 (300 cycles)
TG-142-1001
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Nano Flow Cell (up to 1M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Nano Flow Cell, and Wash Buffer.
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TG MiSeq Reagent Micro Kit v2 (300 cycles)
TG-142-1002
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.
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TG MiSeq Reagent Kit, v2 (300 cycles)
TG-142-1003
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Standard Flow Cell (up to 15M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.
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TG MiSeq Reagent Kit v2 (500 cycles)
TG-142-1013
For TCG customers only - MiSeq v2 Reagent kit, 500 cycles per kit.
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TG MiSeq® Reagent Kit v2 (300 cycles) - 20 Pack
TG-142-1022
For TCG customers only - 20 pack of MiSeq v2 Reagent kits, 300 cycles per kit.
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TG MiSeq Reagent Nano Kit, v2 (300 cycles)
TG-142-1001
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Nano Flow Cell (up to 1M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Nano Flow Cell, and Wash Buffer.
List Price:
Discounts:
TG MiSeq Reagent Micro Kit v2 (300 cycles)
TG-142-1002
Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.
List Price:
Discounts:
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MiSeq Reagent Kit v2 includes pre-filled, ready-to-use reagent cartridges for performing cluster generation and sequencing on the MiSeq System.
There are up to 15M reads per run.
MiSeq Reagent Kit v3 offers a 600-cycle format to enable long read lengths or a 150-cycle format that enables counting applications. MiSeq Reagent Kit v3 contains two additional reagents, the Incorporation Mix (IMT) and Scan Mix (USM), and the PR2 volume has been increased to 500 ml to support longer runs. This ensures double the output of a single run using the v3 kits (3.3–15 Gb) compared to v2 kits (0.75–8.5 Gb).
If you currently own a MiSeq System, you may be eligible for a promotion on one of the following instruments: MiSeq i100 Plus System, NextSeq 1000 System, or NextSeq 2000 System*. Experience a simpler workflow with dry instrumentation, easier run set up, expanded output, and faster secondary analysis. Complete this form to contact a sales representative and see if you are eligible.
* These promotional programs are available for a limited time. Terms and conditions apply.
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Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
This low- to mid-throughput NGS assay enables labs of any size to identify and track the emergence and prevalence of novel SARS-CoV-2 variants.
A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.
A streamlined whole-genome NGS library prep kit for high-impact research and public health surveillance of influenza A and B viruses.
Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.
Looking for more? Reach out for information about our products and services, answers to questions about our technology.
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