Illumina Complete Long Read Prep, Human

Highly accurate, high-performance full workflow solution for comprehensive human WGS with long-read data from NovaSeq platforms. Read More...
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Illumina Complete Long Read Prep, Human (24 samples)

20089108

Illumina Complete Long Read Prep, Human (8 samples)

20086823

Illumina Unique Dual Indexes, LT (48 Indexes, 48 Samples)

20098166

Illumina Complete Long Read DRAGEN Cloud Analysis

20100421

Illumina Complete Long Read Prep, Human - 24, S4 starter pack

20099754

Illumina Complete Long Read Prep, Human - 24, 10B starter pack

20099755

Product Highlights

With Illumina Complete Long Read technology, long-read sequencing is now accessible and streamlined for genomic labs. Illumina Complete Long Read Prep, Human is the first product to utilize this technology, providing the most comprehensive view of the genome. The high-performance whole-genome sequencing assay uses a standard NGS workflow to generate contiguous long-read sequences on the NovaSeq 6000 System and NovaSeq X Series.

  • Streamlined, accessible assay with long and short reads on a single platform, compatible wth the NovaSeq X Series and NovaSeq 6000 System
  • Optimized standard NGS library prep and DRAGEN secondary analysis for highly accurate, reliable results
  • Robust and flexible performance with low input requirements, no specialized extraction, shearing, or size selection required
  • Efficient, single-day, automation-friendly workflow that is more scalable than on-market long-read solutions
  • Note: Analysis requires standard, ≥30× short-read WGS data from the same sample.
Highly accurate, scalable assay

Illumina Complete Long Read data complements standard short-read WGS data and delivers more comprehensive whole genomes by:

  • Calling variants in challenging, challenging-to-map regions with high homology or repetitive regions
  • Resolving complex structural variants, pseudogenes, and large insertion–deletions (indels)
  • Phasing variants and calling haplotypes

This novel technology will bring more light to even the darkest corners of the genome. Illumina Complete Long Reads help resolve the most challenging regions of the genome and makes long-read sequencing accessible and streamlined by enabling short and long reads from a single platform.

View Video
Introducing Illumina Complete Long Read Sequencing Technology

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NovaSeq 6000 System 4 samples (1 sample per lane of an S4 flow cell).
8 samples (1 sample per lane of an S4 flow cell, dual flow cell run optional).
A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended).
NovaSeq 6000 Xp Workflow kit.
2 × 150 bp
NovaSeq 6000Dx Instrument 4 samples (1 sample per lane of an S4 flow cell).
8 samples (1 sample per lane of an S4 flow cell, dual flow cell run optional).
A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended).
NovaSeq 6000 Xp Workflow kit.
2 × 150 bp
NovaSeq X System 4 samples (1 sample per 2 lanes of a 10B flow cell).
A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended).
11 samples (25B flow cell).
2 × 150 bp
NovaSeq X Plus System 8 samples (1 sample per 2 lanes of a 10B flow cell, dual flow cell run).
A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended).
22 samples (25B flow cell, dual flow cell run).
2 × 150 bp

Method-Specific Workflow Example

Supporting Data and Figures

 

In this presentation, we share use cases of Complete Long Reads and highlight research being done by collaborators around the world.

Watch on-demand webinar
Illumina Complete Long-Read technology webinar

Illumina Complete Long Read technology will accelerate access to the remaining ~5% of genic regions. Hear Andrew Shaver, Product Manager describe the benefits of the Illumina novel long-read technology, including the accuracy, scalability, and workflow benefits. See how the Illumina Complete Long Read technology complements the technical innovations in our Illumina product portfolio.

Watch on-demand webinar
Illumina Complete Long-Read technology: Detailed insights into complex genomes

Related Products

Illumina DNA PCR-Free Prep

A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.


NovaSeq 6000 Reagent Kits

Reagent kits for the NovaSeq 6000 system provide ready-to-use cartridge-based reagents for cluster generation and SBS.


NovaSeq X Series Reagent Kits

Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.


Interested in the Illumina Complete Long Read Prep, Human Kit?

Speak with one of our specialists about how your workflow and analysis can benefit from the accuracy and scalability of our long-read technology. Fill out the form to request a quote for ordering today.

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* Sequencing Illumina Complete Long Read libraries on NovaSeq platforms may cause the reported Q30 score of a run to fall below the NovaSeq specification. This does not indicate a performance issue with the sequencing run, nor the library.