Sequencing large genomes (> 5 Mb) can provide valuable information for disease and population-level studies. Researchers often use large whole-genome sequencing to analyze tumors, investigate causes of disease, select plants and animals for agricultural breeding programs, and identify common genetic variations among populations.
Analyzing the whole genome using next-generation sequencing (NGS) delivers a base-by-base view of all genomic alterations, including single nucleotide variants (SNV), insertions and deletions, copy number changes, and structural variations. Paired-end whole-genome sequencing involves sequencing both ends of a DNA fragment, which increases the likelihood of alignment to the reference and facilitates detection of genomic rearrangements, repetitive sequences, and gene fusions.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of global sequencing data.*
In addition to our industry-leading data quality, Illumina offers integrated workflows that simplify the entire process, from library preparation to data analysis.
Click on the below to view products for each workflow step.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.TruSeq DNA PCR-Free Library Prep Kit
Library preparation for whole-genome sequencing of any species without PCR-induced bias.TruSeq Nano DNA Library Prep Kit
Efficient library preparation from samples with limited available DNA, delivering high coverage quality and reduced bias.
Flexible power to sequence the genome, exome, or transcriptome of any species, including up to 1 human genome per run.HiSeq 4000 System
High throughput and low cost for production-scale genomics, sequencing up to 12 large genomes per run.HiSeq X Series
$1000 human genome and ultra-high throughput for population-scale sequencing of up to 18,000 genomes per year.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.Sequencing Platform Comparison Tool
Compare sequencing platforms and identify the best system for your lab and applications.Sequencing Reagents and Flow Cells
Find kits that include sequencing reagents, flow cells, and/or buffers tailored to each Illumina sequencing system.
Quickly extracts biological information from whole-genome sequences, using Isaac alignment and variant calling.BaseSpace Tumor-Normal App
Enables researchers to detect somatic variants from a tumor and matched normal sample pair.BaseSpace Variant Interpreter
Leverages leading annotation databases and a powerful filtering interface to identify disease-associated variants rapidly.
Associates single gene or list of genes with annotation data for pathways, diseases, tissues, and small molecules.BaseSpace Sequence Hub
The Illumina genomics computing environment for NGS data analysis and management.BaseSpace Correlation Engine
A growing library of curated genomic data to support researchers in identifying disease mechanisms, drug targets, and biomarkers.
Whole-genome sequencing of tumor and matched normal tissue samples provides a comprehensive view of the unique mutations in cancer, informing studies of oncogenes and other risk factors. Learn more about cancer genome sequencing.
Whole human genome sequencing can identify single nucleotide variants and copy number variations associated with disease. Identifying these causal variants can help researchers characterize disease mechanisms. Learn more about causal variant discovery.
Whole-genome sequencing of plants and animals is an efficient approach for discovering genes, SNPs, and structural variants. This information can improve breeding and selection in agriculture. Learn more about plant and animal sequencing.
*Data calculations on file. Illumina, Inc., 2015