Sequencing large genomes (> 5 Mb) can provide valuable information for disease and population-level studies. Today, researchers use large whole-genome sequencing to analyze tumors, investigate causes of disease, select plants and animals for agricultural breeding programs, and identify common genetic variations among populations.
Analyzing the whole genome using next-generation sequencing (NGS) delivers a base-by-base view of all genomic alterations, including single nucleotide variants (SNV), insertions and deletions, copy number changes, and structural variations. Paired-end whole-genome sequencing involves sequencing both ends of a DNA fragment, which increases the likelihood of alignment to the reference and facilitates detection of genomic rearrangements, repetitive sequences, and gene fusions.
Library preparation for whole-genome sequencing of any species without PCR-induced bias.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
Push-button extraction of biological data from whole-genome sequences generated using the TruSeq DNA PCR-Free Library Prep Kit to rapidly identify disease-associated variants.
Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of global sequencing data.*
In addition to our industry-leading data quality, Illumina offers integrated workflows that simplify the entire process, from library preparation to data analysis.
Click on the below to view products for each workflow step.
Library preparation for whole-genome sequencing of any species without PCR-induced bias.TruSeq Nano DNA Library Prep Kit
Efficient library preparation from samples with limited available DNA, delivering high coverage quality and reduced bias.Nextera DNA Library Prep Kit
An easy-to-use system for preparing high-quality NGS libraries compatible with all Illumina sequencing platforms.
Gel-free and gel-plus methods for preparing mate pair libraries for sequencing from low DNA input.TruSeq Synthetic Long-Read DNA Library Prep Kit
Highly accurate assembly of long sequencing reads from shorter reads for whole-genome sequencing or genome phasing.Find the Right Whole-Genome Library Prep Kit
Determine the best kit for your needs.
Flexible power to sequence the genome, exome, or transcriptome of any species, including up to 1 human genome per run.HiSeq 2500 System
Production power and efficiency to sequence up to 10 large genomes per run.HiSeq 3000/HiSeq 4000 Systems
Maximum throughput and lowest cost for production-scale genomics, sequencing up to 12 large genomes per run.HiSeq X Series
$1000 human genome and maximum throughput for population-scale sequencing of up to 18,000 genomes per year.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.Platform Comparison Tool
Compare sequencing platforms and identify the best system for your lab and applications.Sequencing Reagents
Find kits that include sequencing reagents, flow cells, and/or buffers tailored to each Illumina sequencing system.
Processes whole-genome sequencing data using BWA alignment and GATK variant calling.Isaac Whole-Genome Sequencing App
Quickly extracts biological information from whole-genome sequences, using Isaac alignment and variant calling.Tumor-Normal App
Detects somatic variants from a tumor and matched normal sample pair.BaseSpace Variant Interpreter
Leverages leading annotation databases and a powerful filtering interface to identify disease-associated variants rapidly.
Associates single gene or list of genes with annotation data for pathways, diseases, tissues, and small molecules.BaseSpace Sequence Hub
The Illumina genomics computing environment for NGS data analysis and management.BaseSpace Correlation Engine
A growing library of curated genomic data to support researchers in identifying disease mechanisms, drug targets, and biomarkers.
Sequencing tumor and matched normal tissue provides a comprehensive view of the unique mutations in cancer, informing studies of oncogenes and other risk factors. Learn more about cancer whole-genome sequencing.
Whole human genome sequencing can identify single nucleotide variants and copy number variations associated with disease. Identifying these causal variants can help researchers characterize disease mechanisms. Learn more about causal variant discovery.
Whole-genome sequencing of plants and animals is an efficient approach for discovering genes, SNPs, and structural variants. This information can improve breeding and selection in agriculture. Learn more about plant and animal sequencing.
*Data calculations on file. Illumina, Inc., 2015