Provides a snapshot of all the ribosomes active in a cell at a specific time point

Ribosome profiling enables systematic monitoring of cellular translation processes and prediction of protein abundance

Ribosome Profiling

Ribosome profiling is a method based on deep sequencing of ribosome-protected mRNA fragments. Purification and sequencing of these fragments provides a “snapshot” of all the ribosomes active in a cell at a specific time point. This information can determine what proteins are being actively translated in a cell.

Ribosome profiling enables systematic monitoring of cellular translation processes and prediction of protein abundance. Determining what regions of a message are being translated can help define the proteome of complex organisms. In combination with massively parallel Illumina high-throughput sequencing, ribosome profiling allows detailed and accurate in vivo analysis of protein production.

Identify transcripts undergoing translation for visibility to an important aspect of gene regulation.

  • Investigate translational control and measure gene expression
  • Identify translation start sites
  • Determine rate of protein synthesis
  • Predict protein abundance
Advantages of Ribosome Profiling

Ribosome profiling uses size-exclusion chromatography. This method streamlines the steps to convert ribosome-protected RNA fragments into libraries compatible with sequencing on Illumina instruments. It is a simple, rapid, and scalable method that does not require special equipment. In contrast, other methods to isolate polysomes and monosomes rely on several hours of ultracentrifugation using either a sucrose gradient or a cushion.

Generating Sequencing Libraries for Ribosome Profiling

Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of sequencing data worldwide.*

In addition to our industry-leading data quality, Illumina offers integrated ribosome profiling workflows that simplify the entire process, from library preparation to data analysis and biological interpretation.

Click on the below to view products for each workflow step.

TruSeq Ribo Profile Library Preparation Kit

Sequence ribosome-protected mRNA fragments from yeast and mammalian samples. No ultracentrifugation required.

Library Prep Kit Selector Library Prep Kit Selector

Determine the best kit for your needs based on project type, starting material, and method or application.

MiSeq Series

Focused power. Speed and simplicity for targeted and small genome sequencing. 1-2 ribosome profiling samples per run.

NextSeq Series

Flexible power. Speed and simplicity for everyday genomics. 7-20 ribosome profiling samples per run.

HiSeq Series

Production power. Max throughput and lowest cost for production-scale genomics. 15-100 ribosome profiling samples per run.

Platform Comparison Tool

Compare sequencing platforms and identify the best system for your lab and applications.

Sequencing Reagents

Find kits that include sequencing reagents, flow cells, and buffers tailored to each Illumina sequencing system.

TruSeq Ribo Profile Bioinformatics Guide

Guidance for software installation, analysis workflows, quality metrics and more.

BaseSpace Sequence Hub

BaseSpace Sequence Hub Apps for RNA-Seq support ribosome profiling experiments.

Cancer Research: Gene Deregulation
Cancer Research: Gene Deregulation

Sequencing ribosome-protected mRNA fragments provides critical insight into gene deregulation in cancer. By measuring the number of ribosomes engaged in translating specific mRNAs, researchers gain insight into functional changes occurring during cancer development. Learn more about cancer gene expression studies.

Complex Disease Research: Translational Regulation
Complex Disease Research: Translational Regulation

Translational control determines the portion of the transcriptome that is undergoing translation. Ribosome profiling further bridges the gap between genomics/transcriptomics and proteomics, providing visibility to an important layer of post-transcriptional regulation and potential new insights into the mechanisms driving complex disease. Learn more about complex disease genomics.

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RNA-Seq With the NextSeq Series
RNA-Seq With the NextSeq Series

The NextSeq Series of sequencing systems offers an accessible, cost-effective RNA-Seq solution.

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RNA-Seq Data Analysis
RNA-Seq Data Analysis

User-friendly BaseSpace Apps simplify RNA-Seq data analysis for the most common RNA studies.

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Key Ribosome Profiling Publications
Key RNA-Seq Publications

View a searchable list of RNA-Seq research publications featuring Illumina technology.

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Ribosome Profiling Enables Full Translation Analysis
Ribosome Profiling Enables Full Translation Analysis

A direct method for analyzing gene expression at the protein synthesis level.

Application Note

*Data calculations on file. Illumina, Inc., 2015