Library preparation is a required sequencing step. Use our selection tool to determine the best library prep kit for your needs based on your application, method, species, and more.
If you currently own a HiSeq, NextSeq 500/550/Dx, or a MiSeq System, you may be eligible to trade in your existing instrument for a NextSeq 1000 or NextSeq 2000 System. Restrictions apply. Contact a sales representative using the form below to learn more about eligibility.
In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.
Data concordance between the NextSeq 1000/2000 and NextSeq 550 Sequencing Systems
Application Note | PDF < 1 MB
Illumina Sequencing Platforms Brochure
Brochure | PDF 4 MB
NextSeq 1000 and NextSeq 2000 single-cell RNA sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 RNA sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
Application Note | PDF < 1 MB
Explore the transcriptome with single-cell resolution
Technical Note | PDF 1 MB
Multiomic interrogation of the immune system at single-cell resolution
Technical Note | PDF 1 MB
Technical Note | PDF | 3 versions
Resolve the whole transcriptome within tissue architecture
Technical Note | PDF 3 MB
Data Sheet | PDF | 8 versions
DRAGEN Bio-IT Platform onboard NextSeq 1000 and NextSeq 2000 systems data sheet
Data Sheet | PDF < 1 MB
High-resolution, high-throughput spatial transcriptomics of complex tissues
Application Note | PDF 3 MB
NextSeq 1000 and 2000 Custom Primers Guide Documentation
Indexed Sequencing Overview Documentation
NextSeq 1000/2000 Sequencing System Guide Documentation
NextSeq 1000 and NextSeq 2000 Sequencing Systems Safety and Compliance Guide Documentation
NextSeq 1000 and 2000 Sequencing System Site Prep Guide Documentation
RFID Reader Compliance Guide Documentation
NextSeq 1000 and 2000 Denature and Dilute Libraries Guide Documentation
NextSeq 1000/2000 Sequencing System Documentation
Security and Networking Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All NextSeq 1000/2000 Sequencing System Support Resources Support
A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA.
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
Highly sensitive detection and characterization of common respiratory viruses, including COVID-19 strains, with comprehensive, rapid target enrichment sequencing.
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with exceptional study flexibility.
