These sequencing systems offer expansive application breadth, operational simplicity, high flexibility and scalability, and proven performance.
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An Illumina Field Applications Scientist discusses what to expect during the preinstallation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.
Find the right library prep kit
Library preparation is a required sequencing step. Use our selection tool to determine the best library prep kit for your needs based on your application, method, species, and more.
Get up and running quickly with a rental or leasing program designed to work with your budget.
Learn why researchers choose the NextSeq 1000 and NextSeq 2000 Systems for efficient, versatile, and easy-to-use sequencing.
Identify the best approach, manage your workflow, purchase what you need, and get support.
NextSeq 2000 Sequencing System
20038897
Illumina NextSeq 2000 Sequencing System is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and secondary analysis. System includes embedded touchscreen monitor and on-instrument computer, control software, hardware accelerated Dragen Bio-IT secondary analysis pipelines, installation and training, and 12 months warranty (including parts and labor).
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NextSeq 1000 Sequencing System
20038898
Illumina NextSeq 1000 Sequencing System is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and secondary analysis. System includes embedded touchscreen monitor and on-instrument computer, control software, hardware accelerated Dragen Bio-IT secondary analysis pipelines, Installation and training, and 12 months warranty (including parts and labor).
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NextSeq 1000 to NextSeq 2000 Upgrade
20047256
The NextSeq 1000 upgrade option enables users to seamlessly upgrade their current NextSeq 1000 instrument to enjoy the benefits of the NextSeq 2000. This includes the ability to run all flow cells currently supported on the NextSeq 2000, including higher output products such as the P3 flow cell.
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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (600 Cycles)
20100981
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 100M single reads).
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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100982
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 100M single reads).
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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100983
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 100M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (600 Cycles)
20100984
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100985
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100986
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100987
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100988
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100989
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100990
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100992
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100993
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100994
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (50 Cycles)
20100995
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 1000/2000 XLEAP-SBS™ Read and Index Primer Kit
20112856
Reagents to utilize custom read and index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 1 custom read primer and 1 custom index primer use
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NextSeq™ 1000/2000 XLEAP-SBS™ Index Primer Kit
20112858
Reagents to utilize custom index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 10 custom index primer uses.
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NextSeq™ 1000/2000 XLEAP-SBS™ Read Primer Kit
20112859
Reagents to utilize custom read primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 10 custom read primer uses.
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NextSeq 1000/2000 P1 Reagents (300 Cycles)
20050264
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq™1000/2000 P1 Reagents(600cycles)
20075294
Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 1000/2000 P2 Reagents (100 Cycles) v3
20046811
Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.
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NextSeq 1000/2000 P2 Reagents (200 Cycles) v3
20046812
Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (200 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.
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NextSeq 1000/2000 P2 Reagents (300 Cycles) v3
20046813
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq™1000/2000 P2 300M Reagents(600cycles)
20075295
Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 300M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (50 Cycles)
20046810
Provides kitted reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (50 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (100 Cycles)
20040559
Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (100 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (200 Cycles)
20040560
Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (200 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (300 Cycles)
20040561
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (300 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 1000/2000 Read and Index Primers
20046115
Reagents to utilize custom read and index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.
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NextSeq 1000/2000 Index Primer Kit
20046116
Reagents to utilize custom index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom index primer uses.
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NextSeq 1000/2000 Read Primer Kit
20046117
Reagents to utilize custom read primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom read primer uses.
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PhiX Control v3
FC-110-3001
Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)
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NextSeq 2000/1000 Single FILTER, IM
20029759
NextSeq 2000/1000 Single FILTER, IM
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The NextSeq 1000 System is a lower cost instrument that runs P1 and P2 flow cells, offering fast turnaround times and lower throughput. The NextSeq 2000 System accesses a wider range of throughputs with P1, P2, P3, and P4 flow cells for more sequencing per sample, larger studies, and better run economics.
XLEAP-SBS chemistry is available on both the NextSeq 1000 and NextSeq 2000 Systems.
Yes, both systems use patterned flow cells to offer high cluster density, reducing the run cost. In these flow cells, monitoring the Cluster PF (%) is a better measure of potential cluster occupancy than measuring cluster density.
NextSeq 1000 and NextSeq 2000 System analysis options include:
Onboard Illumina DRAGEN secondary analysis, included with both systems at no additional cost. DRAGEN analysis provides high-quality variant calls directly off the NextSeq 1000 or NextSeq 2000 System, an intuitive graphical user interface, a streamlined workflow with fewer touchpoints, and fast turnaround time.
DRAGEN pipelines on BaseSpace Sequence Hub and Illumina Connected Analytics
User-created pipelines
If you currently own a HiSeq, NextSeq 500/550, or a MiSeq System, you may be eligible to trade in your existing instrument for a NextSeq 1000 or NextSeq 2000 System. Restrictions apply. Contact a sales representative using the form below to learn more about eligibility.
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Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.
A high-performance, fast, and reliable human whole-exome sequencing solution that includes a comprehensive, up-to-date exome enrichment panel.
These expert-defined sequencing research panels target 94 genes and 284 SNPs associated with a predisposition towards various cancers.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities.
Reach out for information about our products and services, or get answers to questions about our technology.
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