NextSeq 1000/2000 Reagent Kits now offer an extended shelf life of six months with improved Q30 scores.
Order NextSeq 1000 or NextSeq 2000 Sequencing Systems
The NextSeq 1000 and NextSeq 2000 Systems support a broad range of emerging and mid-throughput sequencing applications.
Instrument
Reagents
NextSeq 1000/2000 P1 Reagents (100 cycles)
20074933
Price
Accessory Products
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Find the Right Library Prep Kit
Library preparation is a required sequencing step. Use our selection tool to determine the best library prep kit for your needs based on your application, method, species, and more.
Want to learn more about the NextSeq 1000 and NextSeq 2000 Systems or explore compatible products?
Frequently Purchased Together
Trade In Your HiSeq, MiSeq, or NextSeq 500/550 System
If you currently own a HiSeq, NextSeq 500/550, or a MiSeq System, you may be eligible to trade in your existing instrument for a NextSeq 1000 or NextSeq 2000 System. Restrictions apply. Contact a sales representative using the form below to learn more about eligibility.
Interested in renting or leasing an instrument?
Get up and running quickly with a rental or leasing program designed to work with your budget.
See your financing optionsHow Do I Prepare for My Illumina Instrument Delivery?
In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.
Product Literature
Data concordance between the NextSeq 1000/2000 and NextSeq 550 Sequencing Systems
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 Systems FAQ
FAQ Document | PDF < 1 MB
Brochure | PDF | 5 versions
NextSeq 1000 and NextSeq 2000 single-cell RNA sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 RNA sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
Application Note | PDF < 1 MB
Small whole-genome sequencing on NextSeq 1000 and NextSeq 2000 Systems
Application Note | PDF < 1 MB
16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems
Application Note | PDF < 1 MB
Explore the transcriptome with single-cell resolution
Technical Note | PDF 1 MB
Multiomic interrogation of the immune system at single-cell resolution
Technical Note | PDF 1 MB
Technical Note | PDF | 11 versions
Technical Note | PDF | 2 versions
Technical Note | PDF | 3 versions
Data Sheet | PDF | 5 versions
Data Sheet | PDF | 5 versions
Data Sheet | PDF | 8 versions
High-resolution, high-throughput spatial transcriptomics of complex tissues
Application Note | PDF 3 MB
High-plex spatial proteogenomics of FFPE tissue sections
Application Note | PDF 2 MB
Manuals and Support Information
Indexed Sequencing Overview Documentation
RFID Reader Compliance Guide Documentation
NextSeq 1000/2000 Product Documentation
Illumina Cloud Run Planning Product Documentation
NextSeq 1000/2000 Sequencing System Documentation
Security and Networking Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All NextSeq 1000/2000 Sequencing System Support Resources Support
Illumina Stranded mRNA Prep
A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA.
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
NextSeq 1000/2000 Reagents
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with exceptional study flexibility.
Illumina RNA Prep with Enrichment
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Pan-Coronavirus Panel
This panel allows for characterization of at least 200 known coronavirus genera for surveillance of animal reservoirs and sequencing of known coronaviruses.