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| Max output per flow cell | 540 Gba | 3 Tbb | 8 Tbc |
| Run time (range)d | ~8–44 hr | ~13–44 hr | ~17–48 hr |
| Max reads per run (single reads) | 1.8Ba | 10B (single flow cell)b 20B (dual flow cells)b |
26B (single flow cell)c 52B (dual flow cells)c,e |
| Max read length | 2 × 300 bp | 2 × 250 bp | 2 × 150 bp |
| Key applications and methodsf | |||
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Large whole-genome sequencing (human, plant, animal) |
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Small whole-genome sequencing (microbe, virus) |
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Exome and large panel sequencing (enrichment-based) |
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Targeted gene sequencing (amplicon-based, gene panel) |
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Single-cell profiling (scRNA-Seq, scDNA-Seq, oligo tagging assays) |
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Transcriptome sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling) |
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Chromatin analysis (ATAC-Seq, ChIP-Seq) |
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Methylation sequencing |
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Metagenomic profiling (shotgun metagenomics, metatranscriptomics) |
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Cell-free sequencing & liquid biopsy analysis |
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a. Specifications based on a single flow cell run of P4 flow cell using Illumina PhiX control library at supported cluster densities. P4 flow cells are available for the NextSeq 2000 System only.
b. Specifications based on a single flow cell run of S4 flow cell using Illumina PhiX control library at supported cluster densities.
c. Specifications based on Illumina PhiX control library, or a TruSeq DNA Library created with NA12878 at supported cluster densities. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
d. Run times includes cluster generation, sequencing, and base calling on a NextSeq 1000 or NextSeq 2000 System and NovaSeq 6000 System. Run times include automated onboard cluster generation, sequencing, automated post-run wash, and base calling on the NovaSeq X Systems.
e. Dual flow cell runs only apply to the NovaSeq X Plus System.
f. Checkmark indicates an application or method optimized for the sequencing system.
| Key specifications |
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| Max output per flow cell | 1.2 Gba | 7.5 Gbb | 15 Gbc | 120 Gbb | 540 Gbd |
| Run time (range)e | ~9.5–19 hr | ~5–24 hr | ~5–56 hr | ~11–29 hr | ~8–44 hr |
| Max reads per run (single reads) | 4Ma | 25Mb | 25Mc | 400Mb | 1.8Bd |
| Max read length | 2 × 150 bp | 2 × 150 bp | 2 × 300 bp | 2 × 150 bp | 2 × 300 bp |
a. Specifications are based on the iSeq 100 i1 Reagents v2 (300 cycle) kit using Illumina PhiX control library at supported cluster densities.
b. Specifications are based on a single flow cell run of the high-output flow cell using Illumina PhiX control library at supported cluster densities. MiniSeq and NextSeq 550 Systems use different high-output flow cells.
c. Specifications are based on a single flow cell run of the v3 flow cell using Illumina PhiX control library at supported cluster densities.
d. Specifications based on a single flow cell run of the P4 flow cell using Illumina PhiX control library at supported cluster densities. P4 flow cells are available for the NextSeq 2000 System only.
e. Run times includes cluster generation, sequencing, base calling, and quality scoring.
f. Checkmark indicates an application or method optimized for the sequencing system.
Innovative NGS platforms with proven performance and exceptional customer support expand the genomics community on a global scale. With more than 15 Illumina Solutions Centers worldwide, over 600 support personnel, 24/5 remote technical support, onsite training, instrument qualification services, and customized onboarding solutions, Illumina provides researchers with the tools and support they need to be successful in their lab.
Find the right sequencing instrument by exploring important considerations for every lab.
Which library prep and sequencing methods will you perform for your desired applications? What are your throughput needs?
What are your goals for automating your workflow, from lab management through analysis and interpretation? Are you looking for streamlined comprehensive informatics?
Will you benefit from application flexibility, expert-level technical support, and a global community of scientists?
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