Products for the HiSeq 2500 System

Kits, reagents, accessories, and clinical research products for large-scale genomics

HiSeq 2500 Products

Areas of Research Focus

Product Categories

Nucleic Acid Type

Key Use
Library Preparation Kits
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Nextera DNA Library Preparation Kit

Nextera DNA kits provide a fast and easy library prep workflow, delivering whole-genome sequencing libraries in under 90 min.

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Nextera Mate Pair Library Prep Kit

This library preparation kit offers a gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement.

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Nextera Rapid Capture Custom Enrichment Kit

Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.

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Nextera Rapid Capture Exome and Expanded Exome Kits

Identify coding variants up to 70% faster with this exome library prep and enrichment kit. Expanded kit sequences untranslated regions (UTRs) and miRNA.

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SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.

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TruSeq ChIP Library Preparation Kit

Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.

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TruSeq Custom Amplicon Low Input Kit

This library prep kit delivers sensitive and specific amplicon sequencing results from both low-input and FFPE DNA samples.

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TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons.

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TruSeq DNA Methylation Kit

Converts bisulfite-treated, single-stranded DNA into an Illumina sequencing library. Enables comprehensive whole-genome DNA methylation analysis.

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TruSeq DNA PCR-Free Library Preparation Kit

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.

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TruSeq Methyl Capture EPIC Library Prep Kit

This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.

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TruSeq Nano DNA Library Prep Kit

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.

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TruSeq Nano DNA Library Prep Kit for NeoPrep

DNA library preparation for use with the NeoPrep System, providing comprehensive genomic coverage with low DNA input and minimal hands-on time.

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TruSeq RNA Access Library Prep Kit

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.

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TruSeq RNA Library Prep Kit v2

The TruSeq RNA Library Preparation Kits provide a simple, cost-effective solution for analysis of the coding transcriptome.

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TruSeq Ribo Profile

The TruSeq Ribo Profile kit (formerly ARTseq) sequences ribosome-protected mRNA fragments to provide a snapshot of the active ribosomes in a cell.

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TruSeq Stranded Total RNA Library Prep Kit

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and FFPE.

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TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes rRNA and globin mRNA in a single step.

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TruSeq Stranded mRNA Library Prep Kit

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.

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TruSeq Stranded mRNA Library Prep Kit for NeoPrep

Complete view of the transcriptome with walk-away workflow, lower input requirement, and excellent reproducibility.

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TruSeq Synthetic Long-Read DNA Library Prep Kit

Use this sequencing kit to prepare DNA libraries to generate synthetically long reads, with informatics options for genome assembly or genome phasing.

Cluster Generation and Sequencing Reagents
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HiSeq PE Cluster Kit v4 cBot

This kit uses isothermal amplification to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems.

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HiSeq Rapid Cluster Kit v2 - Paired-End and Single-Read

HiSeq Rapid Cluster Kits v2 enable an on-board cluster generation process, designed to be used in HiSeq Rapid Run mode.

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HiSeq Rapid Duo cBot Sample Loading Kit

The HiSeq Rapid Duo cBot Sample Loading Kit doubles the indexing capability achieved with the on-board cluster generation process alone.

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HiSeq Rapid SBS Kit v2

HiSeq Rapid SBS Kits v2 are designed for the rapid run mode of HiSeq 2500 and 1500 sequencers.

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HiSeq SBS Kit V4

Reagents for determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on enabled HiSeq systems.

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HiSeq SR Cluster Kit v4 cBot

The HiSeq SR (Single Read) Cluster Kit v4 cBot uses isothermal amplification to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems.

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PhiX Control v3

PhiX Control v3 is a ready-to-use control library for Illumina sequencing runs.

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TruSeq Dual Index Sequencing Primer Box

These primers are required to sequence Nextera, TruSeq Synthetic Long-Read DNA, or TruSeq HT libraries in certain cases, as indicated.

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TruSeq PE Cluster Kit v3-cBot-HS

This kit enables cluster generation for subsequent paired-end sequencing on select HiSeq Systems as well as the HiScanSQ System.

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TruSeq Rapid Cluster Kit - Paired-End and Single-Read - HS

TruSeq Rapid Cluster Kits - PE (paired-end) and SR (single-read) - are designed for use in the rapid run mode of HiSeq 2500 and 1500 sequencers.

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TruSeq Rapid Duo cBot Sample Loading Kit

This duo kit enables individually addressable lanes. It requires a cBot and is designed for the rapid run workflow of HiSeq 2500 and 1500 sequencers.

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TruSeq Rapid SBS Kits (200 Cycle and 50 Cycle) - HS

Designed for the rapid run mode of HiSeq 2500 and 1500 sequencers, these kits enable high data quality, fast turnaround time, and a walk-away workflow.

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TruSeq SBS Kit v3-HS

These kits contain ready-to-load reagents to determine the DNA sequence of each cluster on a HiSeq flow cell using sequencing by synthesis technology.

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TruSeq SR Cluster Kit v3-cBot-HS

Use this kit to cluster a flow cell on the cBot for subsequent single-read sequencing in the TruSeq v3 high output mode on enabled HiSeq systems.

Clinical Research Products
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TruSight Inherited Disease

TruSight Inherited Disease is a targeted sequencing panel that focuses on 552 genes associated with severe, recessive pediatric onset diseases.

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TruSight One Sequencing Panel

This panel focuses on exonic regions harboring disease-causing variants. Labs can perform commonly ordered molecular assays on-site with one assay.

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TruSight Rapid Capture

TruSight Rapid Capture all-in-one kits enable targeted sequencing library preparation and enrichment for up to 96 samples in only 1.5 days.

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TruSight Tumor 170

Comprehensive next-generation sequencing (NGS) assay that targets DNA and RNA variants from the same FFPE sample.

Molecular Biology Reagents
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Globin-Zero Gold rRNA Removal Kit

This kit removes unwanted ribosomal RNA and globin mRNA from mammalian blood RNA samples before sequencing.

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Ribo-Zero Gold rRNA Removal Kit (Epidemiology)

This kit removes cytoplasmic and mitochondrial rRNA from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA.

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Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)

This kit removes both cytoplasmic and mitochondrial rRNA from human, mouse, and rat samples, including partially degraded samples.

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Ribo-Zero Gold rRNA Removal Kit (Yeast)

This kit removes both cytoplasmic and mitochondrial ribosomal RNA from yeast samples.

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Ribo-Zero rRNA Removal Kit (Bacteria)

This kit removes ribosomal RNA from Gram-positive and Gram-negative bacterial mixtures in a single pass.

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Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria)

This kit removes unwanted ribosomal RNA from Gram-negative bacterial samples.

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Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria)

This kit removes unwanted ribosomal RNA from Gram-positive bacterial samples.

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Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat)

This kit removes cytoplasmic ribosomal RNA from human, mouse, or rat samples, including partially degraded samples.

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Ribo-Zero rRNA Removal Kit (Plant)

This kit removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA from plant seed, root, or leaf samples in a single pass.

Accessory Products
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cBot 2 Barcoded Strip Tubes

These tubes enable sequencing sample tracking on the cBot 2 automated cluster generation system.

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cBot 2 System

The cBot 2 System streamlines the sequencing workflow by automating cluster generation. It is a required accessory instrument for HiSeq sequencers.