Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria)

This kit removes unwanted ribosomal RNA from Gram-negative bacterial samples. Read More...
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Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) 6 reactions

MRZGN126

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Product Highlights

  • Remove ribosomal RNA (rRNA) from intact and partially degraded Gram (-) bacteria
  • Sequencing data contain complete transcriptome of coding and noncoding RNA species
  • Good for small sample quantities – only 1 μg of total RNA required
  • Nuclear rRNA removed: 23S, 16S, 5S

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with the Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

The Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) offers informative sequencing results by removing unwanted rRNA prior to sequencing. The kit removes rRNA from intact and partially degraded samples.

Removes all major rRNA subunits

The Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) depletes unwanted rRNA from total RNA. Removal of these unwanted subunits produces sequencing results that are heavily focused on biologically informative genomic regions.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads
Step 2: Treat samples with rRNA Removal Solution
Step 3: Remove rRNA
Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kit versions

The Ribo-Zero rRNA Removal Kit (Bacteria) is recommended for samples composed of mixtures of Gram-negative and Gram-positive bacteria.

Related information

rRNA Removal Kit Selection Guide
Ribo-Zero Kit Species Compatibility
Ribo-Zero Kit FAQs

Product Literature

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