Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (to be discontinued)

This kit removes cytoplasmic ribosomal RNA from human, mouse, or rat samples, including partially degraded samples.Read More...
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Ribo-Zero rRNA Removal Kit (H/M/R) 6 reactions


Ribo-Zero rRNA Removal Kit (H/M/R) 24 reactions


Product Highlights

  • Removes ribosomal RNA (rRNA) in a single pass
  • Sequencing data contains complete transcriptome of coding and noncoding RNA species
  • Good for intact and partially degraded small samples – only 1 μg of total RNA required

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with Ribo-Zero (Human/Mouse/Rat) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants and isoforms.

Ribo-Zero (Human/Mouse/Rat) offers informative sequencing results by removing unwanted rRNA prior to sequencing. This kit removes cytoplasmic (nuclear-encoded) rRNAs.

Ribo-Zero retains the complete transcriptome

Ribo-Zero removes rRNA in order to retain the regulatory regions of noncoding RNA, even from low-quality samples. Other methods, such as Poly(A) depletion, do not retain noncoding, regulatory information.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted from intact, degraded, and badly degraded (FFPE) samples in 2 hours.

Step 1: Wash the magnetic beads
Step 2: Treat samples with rRNA Removal Solution
Step 3: Remove rRNA
Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kit versions

The Ribo-Zero Gold (Human/Mouse/Rat) rRNA Removal Kit removes both cytoplasmic and mitochondrial rRNAs, and is compatible with low input samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide
Ribo-Zero Kit Species Compatibility
RNA Matchmaker Tool (test rRNA removal kit compatibility in silico with your species of interest)
Ribo-Zero Kit FAQs

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