5 μg leaf samples of Arabidopsis or Zea mays RNA were treated with Ribo-Zero (Plant Leaf). The samples were then converted into sequencing libraries with ScriptSeq v2, and sequenced on an Illumina sequencing system. Most reads contain biologically informative content.
The Ribo-Zero rRNA Removal Kit (Plant Leaf) and Ribo-Zero rRNA Removal Kit (Plant Seed/Root) remove rRNA from cytoplasmic, chloroplast, and mitochondrial regions. Ribo-Zero (Plant Leaf) contains a higher percentage of chloroplast removal probes (compared to seed/root). Ribo-Zero (Plant Seed/Root) contains a higher percentage of cytoplasmic rRNA removal probes (compared to plant leaf).