Ribo-Zero rRNA Removal Kit (Plant)

This kit removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA from plant seed, root, or leaf samples in a single pass. Read More...
Select Product(s)

Ribo-Zero rRNA Removal Kit (Plant Leaf) 6 reactions

MRZPL116

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Ribo-Zero rRNA Removal Kit (Plant Leaf) 24 reactions

MRZPL1224

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Ribo-Zero rRNA Removal Kit (Plant Seed/Root) 6 reactions

MRZSR116

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Product Highlights

  • Removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA (rRNA) in a single pass
  • Sequencing data contains complete transcriptome of coding and noncoding RNA species
  • Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required
  • Virtually all rRNA is depleted from intact and partially degraded samples in 2 hours.

Sequencing is an important discovery tool for plant research. The sequencing data from samples treated with Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero (Plant Leaf) and Ribo-Zero (Plant Seed/Root) offer informative sequencing results by removing unwanted cytoplasmic, mitochondrial, and chloroplast rRNA prior to sequencing. In order to achieve maximum depletion of rRNA, the Ribo-Zero (Plant Leaf) kit contains a higher percentage of probes to deplete chloroplast rRNA compared to Ribo-Zero (Plant Seed/Root).

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads
Step 2: Treat samples with rRNA Removal Solution
Step 3: Remove rRNA
Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

ScriptSeq Complete Kit (Plant): Recommended for low input plant samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide
Ribo-Zero Kit Species Compatibility
Ribo-Zero Kit FAQs

Supporting Data and Figures

 
Ribosomal RNA Removed
Cytoplasmic Chloroplast Mitochondrial
25S 18S 5.8S 5S 23S 16S 5S 4.5S 18S 5S

The Ribo-Zero rRNA Removal Kit (Plant Leaf) and Ribo-Zero rRNA Removal Kit (Plant Seed/Root) remove rRNA from cytoplasmic, chloroplast, and mitochondrial regions. Ribo-Zero (Plant Leaf) contains a higher percentage of chloroplast removal probes (compared to seed/root). Ribo-Zero (Plant Seed/Root) contains a higher percentage of cytoplasmic rRNA removal probes (compared to plant leaf).

Product Literature

Literature is not currently available for this product.