The HiSeq Rapid Duo cBot Sample Loading Kit allows maximum indexing freedom in rapid run mode by enabling individually addressable lanes. This kit is an optional accessory for HiSeq Rapid Cluster kits (which require a cBot instrument), and is designed for use in the rapid run workflow of HiSeq 2500 and 1500 sequencers.
- Load each flow cell lane independently
- Maximize indexing possibilities
- Separate precious samples physically
Load different samples in each lane
The HiSeq Rapid Duo cBot Sample Loading Kit allows each rapid flow cell lane to be loaded with a unique sample template pool. The duo kit accomplishes individual lane loading by allowing a cBot instrument to be used for the template hybridization and first extension steps of the cluster generation process. To use the cBot to load 2 libraries onto a rapid flow cell, 1 HiSeq Rapid Duo cBot Sample Loading Kit is required. Each kit contains sufficient reagents for loading 1 flow cell.
A specifically designed duo manifold directs each of the 2 samples into the 2 separate rapid flow cell lanes. The cBot portion takes about an hour, after which the flow cell is moved to the HiSeq 2500 or 1500 for further cluster generation steps using a HiSeq Rapid Cluster Kit.
The HiSeq Rapid Duo cBot Sample Loading Kit allows you to use up to 96 unique indexes per lane on the rapid flow cell. This doubles the indexing capability achieved with the on-board cluster generation process alone. Using 2 HiSeq Rapid Duo cBot Sample Loading Kits for 2 rapid flow cells enables up to 384 unique indexes per run on a HiSeq 2500 in rapid run mode.
Keep precious samples separate
For many applications, it's necessary to keep samples physically distinct rather than simply indexing to distinguish one sample from another. For these applications, the HiSeq Rapid Duo cBot Sample Loading Kit offers an ideal solution.